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- W2031445922 abstract "A strain of Penicillium freii was transformed to hygromycin B resistance using the pAN7-1 plasmid. The transformation frequency was approximately 100 transformants per microgram DNA per 1 x 10(7) protoplasts. Southern blotting revealed different types of integration into the genome of P.freii. When non-selectable plasmid DNA was added together with pAN7-1, this resulted in a cotransformation frequency of more than 60%. The cotransformation frequency was estimated using a facile DNA extraction method together with PCR." @default.
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- W2031445922 date "1996-08-01" @default.
- W2031445922 modified "2023-09-27" @default.
- W2031445922 title "Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events" @default.
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- W2031445922 doi "https://doi.org/10.1016/s0944-5013(96)80025-5" @default.
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