FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031475166> ?p ?o ?g. }

• W2031475166 endingPage "248" @default.
• W2031475166 startingPage "238" @default.
• W2031475166 abstract "Evidence for the in vivo transmission of porcine endogenous retrovirus (PERV) from porcine xenografts to various recipient animals has been inconsistent. To characterize the contribution of the host immune system to the potential for PERV transmission from pig islet tissue xenografts to host tissues, we examined two immunoincompetent animal models, thymectomizsed fetal lambs and NODscid mice. Pig proislets were grafted into fetal lambs or adult NODscid mice. Conventional, nested and real-time PCR/RT-PCR tests were used to search for PERV and pig cell-specific sequences (porcine mitochondrial cytochrome oxidase II (COII) or mitochondrial ribosomal 12S) in pig proislets, host liver and spleen at 5-84 days (lambs) or 96 days (mice) after transplantation. Xenografts were harvested at the same time points. The copy number of PERV sequences and host cell-specific nuclear (palmitoylcarnitine transferase) sequences was assessed by real-time PCR to estimate the proportion of PERV-infected host cells. Pig proislets were shown to be PERV+ve by PCR and immunohistochemistry (PERV B env protein p15E). PERV transmission (PERV A, B or C DNA in the absence of porcine COII or 12S sequences) was detected by nested PCR and real-time PCR in 4/12 fetal lamb liver samples 5-23 days after transplantation; the maximum copy number of PERV B env sequences was found at day 5 (700 copies/1 x 10(6) lamb cells). A total of 4/12 fetal lambs demonstrated both PERV and 12S porcine sequences in liver samples (days 5-84) by real-time PCR, suggesting that pig cells had migrated to those tissues and established microchimerism; nested PCR showed evidence for microchimerism (porcine COII sequences alone) in 2/12 lambs (day 5). The incidence of PERV transmission and frequency of microchimerism was similar in host spleen analysed by real-time PCR. Histological examination showed complete xenograft rejection by 23 days after transplantation to fetal lambs. In contrast, pig proislet xenografts survived long term (> or =day 96) in NODscid mice but no PERV transmission was found. Both nested and real-time PCR assays revealed that 2/3 mice had become microchimeric. Long-term expression of PERV A, B and C as well as porcine 12S or COII RNAs was found at the graft site (day 96) only, indicating that PERV transcription and possibly replication, continued in the donor pig islet tissue after transplantation. Overall, detection of PERV transmission and microchimerism was limited by the sensitivity of the PCR assay and the primers chosen. The absence of stable PERV transmission and microchimerism in fetal lambs and the rejection of pig proislet xenografts correlated in time with the establishment of host immunocompetence. We therefore suggest that the frequent failure to identify PERV transmission late after transplantation could be due to the immunological destruction of PERV-infected host cells. Recipient NODscid mice demonstrated long-term microchimerism and intragraft PERV expression, which was consistent with their stable immunoincompetence." @default.
• W2031475166 created "2016-06-24" @default.
• W2031475166 creator A5017767013 @default.
• W2031475166 creator A5020415617 @default.
• W2031475166 creator A5020980488 @default.
• W2031475166 creator A5031490160 @default.
• W2031475166 creator A5039788650 @default.
• W2031475166 creator A5050868002 @default.
• W2031475166 creator A5062759373 @default.
• W2031475166 creator A5064793495 @default.
• W2031475166 date "2007-01-16" @default.
• W2031475166 modified "2023-09-24" @default.
• W2031475166 title "Transient transmission of porcine endogenous retrovirus to fetal lambs after pig islet tissue xenotransplantation" @default.
• W2031475166 cites W135205921 @default.
• W2031475166 cites W1546331692 @default.
• W2031475166 cites W1581414507 @default.
• W2031475166 cites W1967414895 @default.
• W2031475166 cites W1974120320 @default.
• W2031475166 cites W1981275517 @default.
• W2031475166 cites W1986604168 @default.
• W2031475166 cites W1989484846 @default.
• W2031475166 cites W2002129939 @default.
• W2031475166 cites W2003287633 @default.
• W2031475166 cites W2003788960 @default.
• W2031475166 cites W2005965677 @default.
• W2031475166 cites W2008339007 @default.
• W2031475166 cites W2013537164 @default.
• W2031475166 cites W2014154278 @default.
• W2031475166 cites W2014811824 @default.
• W2031475166 cites W2018180123 @default.
• W2031475166 cites W2026285292 @default.
• W2031475166 cites W2028035834 @default.
• W2031475166 cites W2038936010 @default.
• W2031475166 cites W2045134465 @default.
• W2031475166 cites W2048794853 @default.
• W2031475166 cites W2049368361 @default.
• W2031475166 cites W2049943903 @default.
• W2031475166 cites W2055014769 @default.
• W2031475166 cites W2055043387 @default.
• W2031475166 cites W2056812128 @default.
• W2031475166 cites W2057607651 @default.
• W2031475166 cites W2061390129 @default.
• W2031475166 cites W2062490263 @default.
• W2031475166 cites W2063233580 @default.
• W2031475166 cites W2070842365 @default.
• W2031475166 cites W2073847279 @default.
• W2031475166 cites W2075863039 @default.
• W2031475166 cites W2076129653 @default.
• W2031475166 cites W2085446597 @default.
• W2031475166 cites W2085878243 @default.
• W2031475166 cites W2086366727 @default.
• W2031475166 cites W2094254178 @default.
• W2031475166 cites W2108280026 @default.
• W2031475166 cites W2114908540 @default.
• W2031475166 cites W2125841407 @default.
• W2031475166 cites W2131251918 @default.
• W2031475166 cites W2134905547 @default.
• W2031475166 cites W2168021597 @default.
• W2031475166 cites W2282491460 @default.
• W2031475166 cites W4206320024 @default.
• W2031475166 doi "https://doi.org/10.1038/sj.icb.7100028" @default.
• W2031475166 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17228325" @default.
• W2031475166 hasPublicationYear "2007" @default.
• W2031475166 type Work @default.
• W2031475166 sameAs 2031475166 @default.
• W2031475166 citedByCount "21" @default.
• W2031475166 countsByYear W20314751662012 @default.
• W2031475166 countsByYear W20314751662013 @default.
• W2031475166 countsByYear W20314751662014 @default.
• W2031475166 countsByYear W20314751662015 @default.
• W2031475166 countsByYear W20314751662016 @default.
• W2031475166 countsByYear W20314751662022 @default.
• W2031475166 crossrefType "journal-article" @default.
• W2031475166 hasAuthorship W2031475166A5017767013 @default.
• W2031475166 hasAuthorship W2031475166A5020415617 @default.
• W2031475166 hasAuthorship W2031475166A5020980488 @default.
• W2031475166 hasAuthorship W2031475166A5031490160 @default.
• W2031475166 hasAuthorship W2031475166A5039788650 @default.
• W2031475166 hasAuthorship W2031475166A5050868002 @default.
• W2031475166 hasAuthorship W2031475166A5062759373 @default.
• W2031475166 hasAuthorship W2031475166A5064793495 @default.
• W2031475166 hasConcept C104317684 @default.
• W2031475166 hasConcept C121346293 @default.
• W2031475166 hasConcept C126322002 @default.
• W2031475166 hasConcept C141231307 @default.
• W2031475166 hasConcept C153911025 @default.
• W2031475166 hasConcept C159047783 @default.
• W2031475166 hasConcept C16685009 @default.
• W2031475166 hasConcept C172680121 @default.
• W2031475166 hasConcept C203014093 @default.
• W2031475166 hasConcept C2522874641 @default.
• W2031475166 hasConcept C2776185481 @default.
• W2031475166 hasConcept C2778442404 @default.
• W2031475166 hasConcept C2778527173 @default.
• W2031475166 hasConcept C2779234561 @default.
• W2031475166 hasConcept C2780931953 @default.
• W2031475166 hasConcept C2911091166 @default.
• W2031475166 hasConcept C3018622758 @default.

• next