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- W2031567276 abstract "Microbial contaminants were isolated from in vitro callus cultures of garlic Allium sativum L. and Allium longicuspis Regel and identified as Bacillus circulans, Staphylococcus xylosus and Staphylococcus warneri. The isolation procedure was based on microbial enrichment in different media and on microscopic preparations using Gram- and Methylene Blue stainings. Pure cultures of isolates were grown and then identified using commercially available identification kits (API 50 CHB and API Staph) as well as a series of specific physiological tests. Screening experiments with a broad spectrum of antibiotics have shown that the growth inhibition of contaminants was strain-dependent, thereby displaying different minimum inhibitory (bacteriostatic effect) and critical bactericidal concentrations. Phytotoxicity effects and persistence of the antibiotics were examined with a selection of three substances (erythromycin at 10 mg/l, gentamycin at 10 mg/l, imipenem at 5 mg/l) supplemented to the in vitro media and subsequently used for culturing of the calli. Neither phytotoxic effects nor visible bacterial growth (colony formation) were observed with the cultures, but rod-shaped bacteria identified as B. circulans could still be isolated from microbial enrichment samples of the plant material. Although the antibiotics exhibited pronounced bacteriostatic effects, endospores from bacilli may survive such a treatment." @default.
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- W2031567276 date "1996-01-01" @default.
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- W2031567276 title "Identification and antibiotic sensitivity of microbial contaminants from callus cultures of garlic Allium sativum L. and Allium longicuspis Regei" @default.
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- W2031567276 doi "https://doi.org/10.1016/0168-9452(95)04301-2" @default.
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