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- W2031676303 startingPage "7752" @default.
- W2031676303 abstract "ABSTRACT AlgR controls numerous virulence factors in Pseudomonas aeruginosa , including alginate, hydrogen cyanide production, and type IV pilus-mediated twitching motility. In this study, the role of AlgR in biofilms was examined in continuous-flow and static biofilm assays. Strain PSL317 (Δ algR ) produced one-third the biofilm biomass of wild-type strain PAO1. Complementation with algR , but not fimTU-pilVWXY1Y2E , restored PSL317 to the wild-type biofilm phenotype. Comparisons of the transcriptional profiles of biofilm-grown PAO1 and PSL317 revealed that a number of quorum-sensing genes were upregulated in the algR deletion strain. Measurement of rhlA :: lacZ and rhlI :: lacZ promoter fusions confirmed the transcriptional profiling data when PSL317 was grown as a biofilm, but not planktonically. Increased amounts of rhamnolipids and N -butyryl homoserine lactone were detected in the biofilm effluent but not the planktonic supernatants of the algR mutant. Additionally, AlgR specifically bound to the rhlA and rhlI promoters in mobility shift assays. Moreover, PAO1 containing a chromosomal mutated AlgR binding site in its rhlI promoter formed biofilms and produced increased amounts of rhamnolipids similarly to the algR deletion strain. These observations indicate that AlgR specifically represses the Rhl quorum-sensing system during biofilm growth and that such repression is necessary for normal biofilm development. These data also suggest that AlgR may control transcription in a contact-dependent or biofilm-specific manner." @default.
- W2031676303 created "2016-06-24" @default.
- W2031676303 creator A5019294398 @default.
- W2031676303 creator A5036180388 @default.
- W2031676303 creator A5038482435 @default.
- W2031676303 creator A5038510134 @default.
- W2031676303 creator A5055668819 @default.
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- W2031676303 creator A5084034496 @default.
- W2031676303 creator A5089065508 @default.
- W2031676303 creator A5090468448 @default.
- W2031676303 date "2007-11-01" @default.
- W2031676303 modified "2023-09-27" @default.
- W2031676303 title "<i>Pseudomonas aeruginosa</i> AlgR Represses the Rhl Quorum-Sensing System in a Biofilm-Specific Manner" @default.
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