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- W2031693043 abstract "In an attempt to elucidate the possible relationship of hippocalcin to neurological disorders, we isolated and analyzed the human and mouse hippocalcin genes. The human and mouse hippocalcin genes contain three exons and two introns, and span approximately 7 and 8 kb, respectively. The exon/intron splice junctions of the human and mouse genes are all situated in exactly the same position and are not consistently placed with respect to the coding regions of the tandemly repeated EF-hand motifs. The amino acid sequences of human and mouse hippocalcins deduced from the genes are 100% identical. Within the 2-kb 3′-flanking sequences of the human and mouse genes, one conserved polyadenylation signal was identified at positions 762 and 823 bp downstream from TAG, respectively. Within the 2.6-kb 5′-flanking sequences of the human and mouse genes, neither a canonical `TATA' box nor a `CAAT' box was found. Southern blot analysis of the human and mouse genomic DNAs demonstrated that the positive bands coincide exactly with those expected from the sequence of the cloned genes, indicating that the human and mouse hippocalcin genes are present as a single-copy gene. Fluorescence in-situ hybridization revealed that the human hippocalcin gene is located at chromosome 1 p34.2–35 and the mouse hippocalcin gene at chromosome 4 D2–D3." @default.
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- W2031693043 date "1998-12-01" @default.
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- W2031693043 title "Genomic structure and chromosomal mapping of the human and mouse hippocalcin genes" @default.
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- W2031693043 doi "https://doi.org/10.1016/s0378-1119(98)00526-5" @default.
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