FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031744224> ?p ?o ?g. }

• W2031744224 endingPage "189" @default.
• W2031744224 startingPage "173" @default.
• W2031744224 abstract "We report here a novel observation that 2,3,7,8-tetracholorodibenzo-p-dioxin (TCDD) induced predominantly cytochrome P4501A1 (CYP1A1) in rat hepatocytes and predominantly CYP1A2 in human hepatocytes. As part of our research program to evaluate species-differences in response to CYP inducers, we studied the effects of TCDD on CYP1A activity, protein, and gene expression in primary cultures of rat and human hepatocytes. TCDD was found to induce CYP1A activity, measured as ethoxyresorufin-O-deethylase (EROD) activity, in both rat and human hepatocytes. TCDD induction of EROD activity in human hepatocytes (2-5 fold of concurrent solvent control), was significantly lower than that found in rat hepatocytes ( 20-fold of concurrent solvent control). Two structural analogs of TCDD, 2,3,7,8-tetrachlorodibenzofuran (TCDF) and 6-nitro-1,3,8-trichlorodibenzofuran (6-NCDF), were also evaluated. As observed for TCDD, human hepatocytes consistently showed a lower response than rat hepatocytes. As most TCDD-related effects are believed to be mediated via binding of the TCDD-Ah receptor (AhR) complex to DNA, nuclear AhR levels were measured in rat and human hepatocytes after TCDD treatment. We found that the nuclear AhR levels in TCDD-treated rat hepatocytes were approximately 4 times higher than found in TCDD-treated human hepatocytes. However, the estimated binding affinity of [3H]TCDD to nuclear AhR from rat hepatocytes was similar. The species difference in response to TCDD was further evaluated by analysis of CYP1A1 and CYP1A2 mRNA levels using Northern analysis, and P4501A1 and 1A2 protein levels using Western immunoblotting. Results showed that, at both gene expression and protein levels, TCDD induced predominantly CYP1A1 in rat hepatocytes and CYP1A2 in human hepatocytes." @default.
• W2031744224 created "2016-06-24" @default.
• W2031744224 creator A5023781239 @default.
• W2031744224 creator A5028319068 @default.
• W2031744224 creator A5044206316 @default.
• W2031744224 creator A5069018228 @default.
• W2031744224 date "2000-02-01" @default.
• W2031744224 modified "2023-10-17" @default.
• W2031744224 title "2,3,7,8 Tetrachlorodibenzo-p-dioxin induction of cytochrome P4501A in cultured rat and human hepatocytes" @default.
• W2031744224 cites W1485553398 @default.
• W2031744224 cites W1497788006 @default.
• W2031744224 cites W1531649171 @default.
• W2031744224 cites W1569157818 @default.
• W2031744224 cites W1970866430 @default.
• W2031744224 cites W1998781431 @default.
• W2031744224 cites W2014313435 @default.
• W2031744224 cites W2017674715 @default.
• W2031744224 cites W2026959257 @default.
• W2031744224 cites W2029480335 @default.
• W2031744224 cites W2030362999 @default.
• W2031744224 cites W2035806003 @default.
• W2031744224 cites W2038657917 @default.
• W2031744224 cites W2038928623 @default.
• W2031744224 cites W2042872242 @default.
• W2031744224 cites W2052428509 @default.
• W2031744224 cites W2059293500 @default.
• W2031744224 cites W2074933291 @default.
• W2031744224 cites W2088202340 @default.
• W2031744224 cites W2088301481 @default.
• W2031744224 cites W2092938867 @default.
• W2031744224 cites W2130538892 @default.
• W2031744224 cites W2139006314 @default.
• W2031744224 cites W2139508000 @default.
• W2031744224 cites W2162067613 @default.
• W2031744224 cites W2206614915 @default.
• W2031744224 cites W2235410578 @default.
• W2031744224 cites W4293247451 @default.
• W2031744224 doi "https://doi.org/10.1016/s0009-2797(99)00149-0" @default.
• W2031744224 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10728777" @default.
• W2031744224 hasPublicationYear "2000" @default.
• W2031744224 type Work @default.
• W2031744224 sameAs 2031744224 @default.
• W2031744224 citedByCount "79" @default.
• W2031744224 countsByYear W20317442242012 @default.
• W2031744224 countsByYear W20317442242013 @default.
• W2031744224 countsByYear W20317442242014 @default.
• W2031744224 countsByYear W20317442242015 @default.
• W2031744224 countsByYear W20317442242016 @default.
• W2031744224 countsByYear W20317442242017 @default.
• W2031744224 countsByYear W20317442242018 @default.
• W2031744224 countsByYear W20317442242019 @default.
• W2031744224 countsByYear W20317442242020 @default.
• W2031744224 countsByYear W20317442242022 @default.
• W2031744224 countsByYear W20317442242023 @default.
• W2031744224 crossrefType "journal-article" @default.
• W2031744224 hasAuthorship W2031744224A5023781239 @default.
• W2031744224 hasAuthorship W2031744224A5028319068 @default.
• W2031744224 hasAuthorship W2031744224A5044206316 @default.
• W2031744224 hasAuthorship W2031744224A5069018228 @default.
• W2031744224 hasConcept C104317684 @default.
• W2031744224 hasConcept C126322002 @default.
• W2031744224 hasConcept C132154277 @default.
• W2031744224 hasConcept C134018914 @default.
• W2031744224 hasConcept C150194340 @default.
• W2031744224 hasConcept C153911025 @default.
• W2031744224 hasConcept C170493617 @default.
• W2031744224 hasConcept C181199279 @default.
• W2031744224 hasConcept C185592680 @default.
• W2031744224 hasConcept C202751555 @default.
• W2031744224 hasConcept C2225953 @default.
• W2031744224 hasConcept C2776200302 @default.
• W2031744224 hasConcept C33594762 @default.
• W2031744224 hasConcept C526171541 @default.
• W2031744224 hasConcept C54355233 @default.
• W2031744224 hasConcept C55493867 @default.
• W2031744224 hasConcept C62231903 @default.
• W2031744224 hasConcept C71924100 @default.
• W2031744224 hasConcept C81885089 @default.
• W2031744224 hasConcept C86339819 @default.
• W2031744224 hasConcept C86745063 @default.
• W2031744224 hasConcept C86803240 @default.
• W2031744224 hasConceptScore W2031744224C104317684 @default.
• W2031744224 hasConceptScore W2031744224C126322002 @default.
• W2031744224 hasConceptScore W2031744224C132154277 @default.
• W2031744224 hasConceptScore W2031744224C134018914 @default.
• W2031744224 hasConceptScore W2031744224C150194340 @default.
• W2031744224 hasConceptScore W2031744224C153911025 @default.
• W2031744224 hasConceptScore W2031744224C170493617 @default.
• W2031744224 hasConceptScore W2031744224C181199279 @default.
• W2031744224 hasConceptScore W2031744224C185592680 @default.
• W2031744224 hasConceptScore W2031744224C202751555 @default.
• W2031744224 hasConceptScore W2031744224C2225953 @default.
• W2031744224 hasConceptScore W2031744224C2776200302 @default.
• W2031744224 hasConceptScore W2031744224C33594762 @default.
• W2031744224 hasConceptScore W2031744224C526171541 @default.
• W2031744224 hasConceptScore W2031744224C54355233 @default.
• W2031744224 hasConceptScore W2031744224C55493867 @default.
• W2031744224 hasConceptScore W2031744224C62231903 @default.

• next