FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031745451> ?p ?o ?g. }

• W2031745451 endingPage "471" @default.
• W2031745451 startingPage "462" @default.
• W2031745451 abstract "A cotton (Gossypium hirsutum L.) genomic clone encompassing a 17.9-kb DNA fragment was found to contain a delta-12 fatty acid desaturase gene (designated FAD2-4). The FAD2-4 open reading frame has 1,155bp and is uninterrupted, encoding a conceptual FAD2-4 polypeptide of 384 amino acids that has 98% identity with the cotton FAD2-3 polypeptide. The FAD2-4 gene has a single intron of 2,780 bp in its 5'-untranslated region (5'-UTR). The 3'-flanking regions and 5'-UTR introns in the FAD2-4 and FAD2-3 genes are quite different, indicating that the genes might be paralogs in the cotton genome. Reverse transcriptase (RT)-PCR analysis indicated that the FAD2-4 and FAD2-3 genes were expressed in all tissues examined, including seeds, seedling tissues, young and mature leaves, roots, stems, developing flower buds, and ovule fibers. These constitutive patterns of expression were notably different from that of the FAD2-1 gene, which was restricted to seeds and developing flower buds, or to the expression of a newly-identified FAD2-2 gene isoform, which was barely detectable in roots, hypocotyls, stems, and fibers, but was expressed in all other tissues. The FAD2-4 coding region was expressed in yeast and shown to encode a functional delta-12 desaturase, converting oleic acid (C18:1) to linoleic acid (C18:2) in recombinant yeast cells. In addition, both the FAD2-4 and the FAD2-3 genes were transferred into the Arabidopsis thaliana fad2-1 mutant background where they effectively restored wild type fatty acid composition and growth characteristics. Finally, the cotton FAD2-4 green fluorescent protein (GFP) fusion polypeptide appeared to be localized in the endomembrane system of transgenic Arabidopsis plants in the complemented fad2-1 mutant background, supporting a functional ER location for the cotton FAD2-4 polypeptide in this heterologous plant system. Thus, a new functional member of the FAD2 gene family in cotton has been characterized, indicating a complex regulation of membrane lipid desaturation in this important fiber/oilseed crop." @default.
• W2031745451 created "2016-06-24" @default.
• W2031745451 creator A5002686497 @default.
• W2031745451 creator A5015580792 @default.
• W2031745451 creator A5021739134 @default.
• W2031745451 creator A5022642575 @default.
• W2031745451 creator A5047695847 @default.
• W2031745451 creator A5064777113 @default.
• W2031745451 creator A5071804203 @default.
• W2031745451 creator A5088971350 @default.
• W2031745451 date "2009-06-01" @default.
• W2031745451 modified "2023-09-25" @default.
• W2031745451 title "Identification and expression of a new delta-12 fatty acid desaturase (FAD2-4) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants" @default.
• W2031745451 cites W1539018267 @default.
• W2031745451 cites W1567397160 @default.
• W2031745451 cites W1583821488 @default.
• W2031745451 cites W1964323757 @default.
• W2031745451 cites W1967390261 @default.
• W2031745451 cites W1967691407 @default.
• W2031745451 cites W1984293833 @default.
• W2031745451 cites W1987186699 @default.
• W2031745451 cites W1990978381 @default.
• W2031745451 cites W1999268654 @default.
• W2031745451 cites W2003041228 @default.
• W2031745451 cites W2009230198 @default.
• W2031745451 cites W2014669590 @default.
• W2031745451 cites W2016993318 @default.
• W2031745451 cites W2020669622 @default.
• W2031745451 cites W2022714377 @default.
• W2031745451 cites W2024236604 @default.
• W2031745451 cites W2032853804 @default.
• W2031745451 cites W2046404691 @default.
• W2031745451 cites W2076543049 @default.
• W2031745451 cites W2080163922 @default.
• W2031745451 cites W2092686336 @default.
• W2031745451 cites W2099626579 @default.
• W2031745451 cites W2103908409 @default.
• W2031745451 cites W2106319097 @default.
• W2031745451 cites W2106629711 @default.
• W2031745451 cites W2113701786 @default.
• W2031745451 cites W2116122104 @default.
• W2031745451 cites W2131518084 @default.
• W2031745451 cites W2142980726 @default.
• W2031745451 cites W2143629303 @default.
• W2031745451 cites W2150816271 @default.
• W2031745451 cites W2151221069 @default.
• W2031745451 cites W2153328054 @default.
• W2031745451 cites W2160973013 @default.
• W2031745451 cites W2163448850 @default.
• W2031745451 cites W2174770349 @default.
• W2031745451 cites W301542239 @default.
• W2031745451 cites W4247997175 @default.
• W2031745451 doi "https://doi.org/10.1016/j.plaphy.2008.12.024" @default.
• W2031745451 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19217793" @default.
• W2031745451 hasPublicationYear "2009" @default.
• W2031745451 type Work @default.
• W2031745451 sameAs 2031745451 @default.
• W2031745451 citedByCount "74" @default.
• W2031745451 countsByYear W20317454512012 @default.
• W2031745451 countsByYear W20317454512013 @default.
• W2031745451 countsByYear W20317454512014 @default.
• W2031745451 countsByYear W20317454512015 @default.
• W2031745451 countsByYear W20317454512016 @default.
• W2031745451 countsByYear W20317454512017 @default.
• W2031745451 countsByYear W20317454512018 @default.
• W2031745451 countsByYear W20317454512019 @default.
• W2031745451 countsByYear W20317454512020 @default.
• W2031745451 countsByYear W20317454512021 @default.
• W2031745451 countsByYear W20317454512022 @default.
• W2031745451 countsByYear W20317454512023 @default.
• W2031745451 crossrefType "journal-article" @default.
• W2031745451 hasAuthorship W2031745451A5002686497 @default.
• W2031745451 hasAuthorship W2031745451A5015580792 @default.
• W2031745451 hasAuthorship W2031745451A5021739134 @default.
• W2031745451 hasAuthorship W2031745451A5022642575 @default.
• W2031745451 hasAuthorship W2031745451A5047695847 @default.
• W2031745451 hasAuthorship W2031745451A5064777113 @default.
• W2031745451 hasAuthorship W2031745451A5071804203 @default.
• W2031745451 hasAuthorship W2031745451A5088971350 @default.
• W2031745451 hasConcept C104317684 @default.
• W2031745451 hasConcept C143065580 @default.
• W2031745451 hasConcept C150194340 @default.
• W2031745451 hasConcept C19038510 @default.
• W2031745451 hasConcept C2775988993 @default.
• W2031745451 hasConcept C2777129432 @default.
• W2031745451 hasConcept C2779491563 @default.
• W2031745451 hasConcept C2780809830 @default.
• W2031745451 hasConcept C543025807 @default.
• W2031745451 hasConcept C54355233 @default.
• W2031745451 hasConcept C55493867 @default.
• W2031745451 hasConcept C86803240 @default.
• W2031745451 hasConcept C94671646 @default.
• W2031745451 hasConceptScore W2031745451C104317684 @default.
• W2031745451 hasConceptScore W2031745451C143065580 @default.
• W2031745451 hasConceptScore W2031745451C150194340 @default.
• W2031745451 hasConceptScore W2031745451C19038510 @default.
• W2031745451 hasConceptScore W2031745451C2775988993 @default.
• W2031745451 hasConceptScore W2031745451C2777129432 @default.

• next