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- W2031821570 abstract "To investigate the influence of neurotransmitter on G-protein-coupled receptor trafficking and compartimentalization in neurons, we have developed a model of primary neuronal cultures from fetal rat striatum on which we have studied the cellular and subcellular distribution and trafficking of the D1 dopaminergic receptor. This receptor is known to be somatodendritic and axonal targeted in vivo, mostly to extrasynaptic locations. Immunohistochemical studies at the light and electron microscopic levels showed that, in cultures, the D1 dopaminergic receptor is expressed in the absence of dopamine stimulation. The pattern of D1 dopaminergic receptor immunostaining after stimulation by the D1 dopaminergic receptor agonist SKF 82958 (1 μM) is dramatically modified with a decrease of the number of labeled D1 dopaminergic receptor puncta (−40%) and an increase of their size in both dendrites (+120%) and axons (+240%). Seven hours after removal of the agonist, return to normal pattern was observed. The D1 dopaminergic receptor antagonist SCH 23390 (2 μM) abolishes the effect of SKF 82958. Electron microscopy demonstrated, in dendrites, a translocation of the labeling from the plasma membrane to endosomes. Axonal D1 dopaminergic receptor redistribution after acute stimulation indicates that the D1 dopaminergic receptor is membrane targeted and responsive to stimulation. These results validate primary culture of striatal neurons to study subcellular localization and intraneuronal trafficking of G-protein-coupled receptors. This preparation will be useful to address various questions concerning the behavior and the trafficking of these receptors in neurons in relation to the neurotransmitter environment." @default.
- W2031821570 created "2016-06-24" @default.
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- W2031821570 date "2000-09-01" @default.
- W2031821570 modified "2023-10-16" @default.
- W2031821570 title "Agonist stimulation provokes dendritic and axonal dopamine D1 receptor redistribution in primary cultures of striatal neurons" @default.
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- W2031821570 doi "https://doi.org/10.1016/s0306-4522(00)00187-1" @default.
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