Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031845110> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2031845110 endingPage "651" @default.
- W2031845110 startingPage "643" @default.
- W2031845110 abstract "Use of the fluorescent base analogue 2-aminopurine has provided a direct demonstration of the translocation of PcrA helicase toward the 5‘-end of single-stranded DNA. Single 2-aminopurine bases are introduced into otherwise standard oligonucleotides and produce a fluorescence signal when PcrA reaches their position. We demonstrate that random binding of PcrA to ssDNA is followed by translocation in an ATP-dependent manner toward the 5‘-terminus at 80 bases per second at 20 °C. The data also provide information on the kinetics of ssDNA binding to the helicase and of the protein dissociation from the 5‘-end of ssDNA. A full kinetic model is presented for ATP-dependent DNA translocation by PcrA helicase." @default.
- W2031845110 created "2016-06-24" @default.
- W2031845110 creator A5052181591 @default.
- W2031845110 creator A5055389331 @default.
- W2031845110 creator A5078281981 @default.
- W2031845110 date "2001-12-13" @default.
- W2031845110 modified "2023-09-25" @default.
- W2031845110 title "Direct Measurement of Single-Stranded DNA Translocation by PcrA Helicase Using the Fluorescent Base Analogue 2-Aminopurine" @default.
- W2031845110 cites W1836579195 @default.
- W2031845110 cites W1974784567 @default.
- W2031845110 cites W2005700199 @default.
- W2031845110 cites W2018325248 @default.
- W2031845110 cites W2048680704 @default.
- W2031845110 cites W2065679107 @default.
- W2031845110 cites W2122571070 @default.
- W2031845110 cites W2135015314 @default.
- W2031845110 cites W2143181513 @default.
- W2031845110 cites W2462622511 @default.
- W2031845110 doi "https://doi.org/10.1021/bi011137k" @default.
- W2031845110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11781105" @default.
- W2031845110 hasPublicationYear "2001" @default.
- W2031845110 type Work @default.
- W2031845110 sameAs 2031845110 @default.
- W2031845110 citedByCount "102" @default.
- W2031845110 countsByYear W20318451102012 @default.
- W2031845110 countsByYear W20318451102013 @default.
- W2031845110 countsByYear W20318451102014 @default.
- W2031845110 countsByYear W20318451102015 @default.
- W2031845110 countsByYear W20318451102016 @default.
- W2031845110 countsByYear W20318451102017 @default.
- W2031845110 countsByYear W20318451102018 @default.
- W2031845110 countsByYear W20318451102019 @default.
- W2031845110 countsByYear W20318451102020 @default.
- W2031845110 countsByYear W20318451102021 @default.
- W2031845110 countsByYear W20318451102022 @default.
- W2031845110 crossrefType "journal-article" @default.
- W2031845110 hasAuthorship W2031845110A5052181591 @default.
- W2031845110 hasAuthorship W2031845110A5055389331 @default.
- W2031845110 hasAuthorship W2031845110A5078281981 @default.
- W2031845110 hasConcept C104317684 @default.
- W2031845110 hasConcept C121332964 @default.
- W2031845110 hasConcept C12554922 @default.
- W2031845110 hasConcept C129312508 @default.
- W2031845110 hasConcept C138626823 @default.
- W2031845110 hasConcept C161223559 @default.
- W2031845110 hasConcept C185592680 @default.
- W2031845110 hasConcept C552990157 @default.
- W2031845110 hasConcept C55493867 @default.
- W2031845110 hasConcept C62520636 @default.
- W2031845110 hasConcept C67705224 @default.
- W2031845110 hasConcept C86803240 @default.
- W2031845110 hasConcept C91881484 @default.
- W2031845110 hasConcept C95444343 @default.
- W2031845110 hasConceptScore W2031845110C104317684 @default.
- W2031845110 hasConceptScore W2031845110C121332964 @default.
- W2031845110 hasConceptScore W2031845110C12554922 @default.
- W2031845110 hasConceptScore W2031845110C129312508 @default.
- W2031845110 hasConceptScore W2031845110C138626823 @default.
- W2031845110 hasConceptScore W2031845110C161223559 @default.
- W2031845110 hasConceptScore W2031845110C185592680 @default.
- W2031845110 hasConceptScore W2031845110C552990157 @default.
- W2031845110 hasConceptScore W2031845110C55493867 @default.
- W2031845110 hasConceptScore W2031845110C62520636 @default.
- W2031845110 hasConceptScore W2031845110C67705224 @default.
- W2031845110 hasConceptScore W2031845110C86803240 @default.
- W2031845110 hasConceptScore W2031845110C91881484 @default.
- W2031845110 hasConceptScore W2031845110C95444343 @default.
- W2031845110 hasIssue "2" @default.
- W2031845110 hasLocation W20318451101 @default.
- W2031845110 hasLocation W20318451102 @default.
- W2031845110 hasOpenAccess W2031845110 @default.
- W2031845110 hasPrimaryLocation W20318451101 @default.
- W2031845110 hasRelatedWork W1963485674 @default.
- W2031845110 hasRelatedWork W1986663423 @default.
- W2031845110 hasRelatedWork W2007063699 @default.
- W2031845110 hasRelatedWork W2021665153 @default.
- W2031845110 hasRelatedWork W2031845110 @default.
- W2031845110 hasRelatedWork W2051959261 @default.
- W2031845110 hasRelatedWork W2052341553 @default.
- W2031845110 hasRelatedWork W2093323007 @default.
- W2031845110 hasRelatedWork W4211158745 @default.
- W2031845110 hasRelatedWork W4293485588 @default.
- W2031845110 hasVolume "41" @default.
- W2031845110 isParatext "false" @default.
- W2031845110 isRetracted "false" @default.
- W2031845110 magId "2031845110" @default.
- W2031845110 workType "article" @default.