Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031874005> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2031874005 endingPage "15875" @default.
- W2031874005 startingPage "15867" @default.
- W2031874005 abstract "To find a disulfide pair that could stabilize the enzyme human carbonic anhydrase II (HCA II), we grafted the disulfide bridge from the related and unusually stable carbonic anhydrase form from Neisseria gonorrhoeae (NGCA) into the human enzyme. Thus, the two Cys residues at positions 23 and 203 were engineered into a pseudo-wild-type form of HCA II (C206S), giving the mutant C206S/A23C/L203C. The disulfide bond was not formed spontaneously. The native state of the reduced form of the mutant was markedly destabilized (2.9 kcal/mol) compared to that of HCA II. Formation of a disulfide bridge was achieved by treatment by oxidized glutathione. This led to a significant stabilization of the native conformation. Compared to HCA II the unfolding midpoint for the variant was increased from 0.9 to 1.7 M guanidine HCl, corresponding to a stabilization of 3.7 kcal/mol. This makes the human enzyme almost as stable as the model protein NGCA, for which the unfolding of the native state has a midpoint at 2.1 M guanidine HCl. The stabilized protein underwent, contrary to all other investigated variants of HCA II, an apparent two-state unfolding transition, as judged from intrinsic Trp fluorescence measurements. A molten-globule intermediate is nevertheless formed but is suppressed because of the high denaturant pressure it faces upon rupture of the native state." @default.
- W2031874005 created "2016-06-24" @default.
- W2031874005 creator A5009175668 @default.
- W2031874005 creator A5013834451 @default.
- W2031874005 creator A5079241780 @default.
- W2031874005 date "2002-12-01" @default.
- W2031874005 modified "2023-10-17" @default.
- W2031874005 title "Dramatic Stabilization of the Native State of Human Carbonic Anhydrase II by an Engineered Disulfide Bond" @default.
- W2031874005 cites W1022983837 @default.
- W2031874005 cites W1525083915 @default.
- W2031874005 cites W1573899446 @default.
- W2031874005 cites W1964570752 @default.
- W2031874005 cites W1971185623 @default.
- W2031874005 cites W1983659508 @default.
- W2031874005 cites W1995215114 @default.
- W2031874005 cites W2009466851 @default.
- W2031874005 cites W2020520177 @default.
- W2031874005 cites W2021631641 @default.
- W2031874005 cites W2035046825 @default.
- W2031874005 cites W2040301369 @default.
- W2031874005 cites W2048466571 @default.
- W2031874005 cites W2048478260 @default.
- W2031874005 cites W2057183688 @default.
- W2031874005 cites W2071155271 @default.
- W2031874005 cites W2090338919 @default.
- W2031874005 cites W2240879578 @default.
- W2031874005 cites W2941501468 @default.
- W2031874005 doi "https://doi.org/10.1021/bi020433+" @default.
- W2031874005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12501217" @default.
- W2031874005 hasPublicationYear "2002" @default.
- W2031874005 type Work @default.
- W2031874005 sameAs 2031874005 @default.
- W2031874005 citedByCount "35" @default.
- W2031874005 countsByYear W20318740052012 @default.
- W2031874005 countsByYear W20318740052013 @default.
- W2031874005 countsByYear W20318740052014 @default.
- W2031874005 countsByYear W20318740052015 @default.
- W2031874005 countsByYear W20318740052016 @default.
- W2031874005 countsByYear W20318740052018 @default.
- W2031874005 countsByYear W20318740052019 @default.
- W2031874005 countsByYear W20318740052023 @default.
- W2031874005 crossrefType "journal-article" @default.
- W2031874005 hasAuthorship W2031874005A5009175668 @default.
- W2031874005 hasAuthorship W2031874005A5013834451 @default.
- W2031874005 hasAuthorship W2031874005A5079241780 @default.
- W2031874005 hasConcept C118493188 @default.
- W2031874005 hasConcept C133571119 @default.
- W2031874005 hasConcept C181199279 @default.
- W2031874005 hasConcept C185592680 @default.
- W2031874005 hasConcept C19472624 @default.
- W2031874005 hasConcept C27256138 @default.
- W2031874005 hasConcept C2775914622 @default.
- W2031874005 hasConcept C2779463914 @default.
- W2031874005 hasConcept C2780416268 @default.
- W2031874005 hasConcept C55493867 @default.
- W2031874005 hasConcept C71240020 @default.
- W2031874005 hasConcept C8010536 @default.
- W2031874005 hasConceptScore W2031874005C118493188 @default.
- W2031874005 hasConceptScore W2031874005C133571119 @default.
- W2031874005 hasConceptScore W2031874005C181199279 @default.
- W2031874005 hasConceptScore W2031874005C185592680 @default.
- W2031874005 hasConceptScore W2031874005C19472624 @default.
- W2031874005 hasConceptScore W2031874005C27256138 @default.
- W2031874005 hasConceptScore W2031874005C2775914622 @default.
- W2031874005 hasConceptScore W2031874005C2779463914 @default.
- W2031874005 hasConceptScore W2031874005C2780416268 @default.
- W2031874005 hasConceptScore W2031874005C55493867 @default.
- W2031874005 hasConceptScore W2031874005C71240020 @default.
- W2031874005 hasConceptScore W2031874005C8010536 @default.
- W2031874005 hasIssue "52" @default.
- W2031874005 hasLocation W20318740051 @default.
- W2031874005 hasLocation W20318740052 @default.
- W2031874005 hasOpenAccess W2031874005 @default.
- W2031874005 hasPrimaryLocation W20318740051 @default.
- W2031874005 hasRelatedWork W1964271368 @default.
- W2031874005 hasRelatedWork W1993736519 @default.
- W2031874005 hasRelatedWork W1996920925 @default.
- W2031874005 hasRelatedWork W2012370493 @default.
- W2031874005 hasRelatedWork W2026924497 @default.
- W2031874005 hasRelatedWork W2031874005 @default.
- W2031874005 hasRelatedWork W2035851218 @default.
- W2031874005 hasRelatedWork W2057183688 @default.
- W2031874005 hasRelatedWork W2073416577 @default.
- W2031874005 hasRelatedWork W2415010756 @default.
- W2031874005 hasVolume "41" @default.
- W2031874005 isParatext "false" @default.
- W2031874005 isRetracted "false" @default.
- W2031874005 magId "2031874005" @default.
- W2031874005 workType "article" @default.