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- W203197195 abstract "It was demonstrated that an increase in malt extract from three to five per cent in agar pluginocula with two per cent agar enabled Hypoxylon pruinatum (Klotzsche) Cke. to colonize plates ofmalt agar (2 per cent agar and 3 per cent malt extract) containing 900 p.p.m. of pyrocatechol. Furthermore, agar plug inocula in contact with test media affected the accuracy of cultural testsof inhibition of fungous pathogens by chemicals. For example, mycelial growth rather than totalinhibition was evident in the same plates when agar plug inocula were placed on and near themargin of sterile cover slips on test media, thus preventing direct contact with the test media. Apparently, in these instances, the total inhibition of fungi was related to type of inoculum ratherto concentration of fungistatic substances in the media. The development of H. pruinatum from bark meal (Populus tremuloides Michx.) inoculum was superior to that from agar plug inoculum on sterile substrates containing different concentrations of pyrocatechol. The maximum growthof the fungus occurred when infected portions of untreated bark meal were used as inoculum. The non-inhibiting bark saprophytes associated with Hypoxylon in this type of inoculum appeared to interact with the pyrocatechol and result in favorable substrates for the growth of thepathogen. Pathogenicity tests of H. pruinatum on living, untreated cuttings of P. tremuloides were positive indicating canker susceptibility when the mycelial inoculum consisted of agar plugs containing 2 per cent agar and 5 per cent malt extract, or infected portions of autoclaved bark meal of thehost. However, negative inoculations, indicating canker resistance, were obtained on the samecuttings when agar plug inoculum containing 2 per cent agar and 3 per cent malt extract wereplaced in the wounds. Mycelium of the pathogen failed to become established in the woundstreated with this type of inoculum and, frequently, the agar plugs became contaminated withbark saprophytes. After a period of seven days, abundant callous tissue was evident at the marginof most of these wounds. It was concluded that problems related to inoculum potential may have been of critical importance to the results obtained in the pathogenicity tests." @default.
- W203197195 created "2016-06-24" @default.
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- W203197195 date "1966-01-01" @default.
- W203197195 modified "2023-09-27" @default.
- W203197195 title "SOME PROBLEMS OF INOCULUM POTENTIAL IN CULTURAL AND PATHOGENICITY TESTS RELATED TO DISEASE RESISTANCE" @default.
- W203197195 cites W1998641535 @default.
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- W203197195 doi "https://doi.org/10.1016/b978-0-08-011764-5.50081-x" @default.
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