FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032014069> ?p ?o ?g. }

• W2032014069 abstract "Abstract Background Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins), composed of Gα, Gβ, and Gγ subunits, are positioned at the inner face of the plasma membrane and relay signals from activated G protein-coupled cell surface receptors to various signaling pathways. Gβ5 is the most structurally divergent Gβ isoform and forms tight heterodimers with regulator of G protein signalling (RGS) proteins of the R7 subfamily (R7-RGS). The subcellular localization of Gβ 5/R7-RGS protein complexes is regulated by the palmitoylation status of the associated R7-binding protein (R7BP), a recently discovered SNARE-like protein. We investigate here whether R7BP controls the targeting of Gβ5/R7-RGS complexes to lipid rafts, cholesterol-rich membrane microdomains where conventional heterotrimeric G proteins and some effector proteins are concentrated in neurons and brain. Results We show that endogenous Gβ5/R7-RGS/R7BP protein complexes are present in native neuron-like PC12 cells and that a fraction is targeted to low-density, detergent-resistant membrane lipid rafts. The buoyant density of endogenous raft-associated Gβ5/R7-RGS protein complexes in PC12 cells was similar to that of lipid rafts containing the palmitoylated marker proteins PSD-95 and LAT, but distinct from that of the membrane microdomain where flotillin was localized. Overexpression of wild-type R7BP, but not its palmitoylation-deficient mutant, greatly enriched the fraction of endogenous Gβ5/R7-RGS protein complexes in the lipid rafts. In HEK-293 cells the palmitoylation status of R7BP also regulated the lipid raft targeting of co-expressed Gβ5/R7-RGS/R7BP proteins. A fraction of endogenous Gβ5/R7-RGS/R7BP complexes was also present in lipid rafts in mouse brain. Conclusion A fraction of Gβ5/R7-RGS/R7BP protein complexes is targeted to low-density, detergent-resistant membrane lipid rafts in PC12 cells and brain. In cultured cells, the palmitoylation status of R7BP regulated the lipid raft targeting of endogenous or co-expressed Gβ5/R7-RGS proteins. Taken together with recent evidence that the kinetic effects of the Gβ5 complex on GPCR signaling are greatly enhanced by R7BP palmitoylation through a membrane-anchoring mechanism, our data suggest the targeting of the Gβ5/R7-RGS/R7BP complex to lipid rafts in neurons and brain, where G proteins and their effectors are concentrated, may be central to the G protein regulatory function of the complex." @default.
• W2032014069 created "2016-06-24" @default.
• W2032014069 creator A5007107739 @default.
• W2032014069 creator A5022331909 @default.
• W2032014069 creator A5054219492 @default.
• W2032014069 creator A5072284526 @default.
• W2032014069 creator A5076718607 @default.
• W2032014069 creator A5084187130 @default.
• W2032014069 date "2007-09-19" @default.
• W2032014069 modified "2023-09-27" @default.
• W2032014069 title "R7-binding protein targets the G protein β5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain" @default.
• W2032014069 cites W1520928812 @default.
• W2032014069 cites W1540870240 @default.
• W2032014069 cites W1557138289 @default.
• W2032014069 cites W1564861448 @default.
• W2032014069 cites W1852817901 @default.
• W2032014069 cites W1965819474 @default.
• W2032014069 cites W1975651701 @default.
• W2032014069 cites W1977085484 @default.
• W2032014069 cites W1977202617 @default.
• W2032014069 cites W1978686382 @default.
• W2032014069 cites W1980926257 @default.
• W2032014069 cites W1990524685 @default.
• W2032014069 cites W2016923687 @default.
• W2032014069 cites W2032582749 @default.
• W2032014069 cites W2046715091 @default.
• W2032014069 cites W2048559110 @default.
• W2032014069 cites W2051549095 @default.
• W2032014069 cites W2053674805 @default.
• W2032014069 cites W2057906276 @default.
• W2032014069 cites W2062243309 @default.
• W2032014069 cites W2062416537 @default.
• W2032014069 cites W2062418569 @default.
• W2032014069 cites W2065128867 @default.
• W2032014069 cites W2065968830 @default.
• W2032014069 cites W2066887588 @default.
• W2032014069 cites W2067827045 @default.
• W2032014069 cites W2068146930 @default.
• W2032014069 cites W2069629128 @default.
• W2032014069 cites W2070194395 @default.
• W2032014069 cites W2077856147 @default.
• W2032014069 cites W2085708545 @default.
• W2032014069 cites W2089844067 @default.
• W2032014069 cites W2106492868 @default.
• W2032014069 cites W2126176654 @default.
• W2032014069 cites W2137144754 @default.
• W2032014069 cites W2142475134 @default.
• W2032014069 cites W2143213067 @default.
• W2032014069 cites W2331959327 @default.
• W2032014069 cites W1482240896 @default.
• W2032014069 doi "https://doi.org/10.1186/1471-2091-8-18" @default.
• W2032014069 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2048962" @default.
• W2032014069 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17880698" @default.
• W2032014069 hasPublicationYear "2007" @default.
• W2032014069 type Work @default.
• W2032014069 sameAs 2032014069 @default.
• W2032014069 citedByCount "13" @default.
• W2032014069 countsByYear W20320140692012 @default.
• W2032014069 countsByYear W20320140692016 @default.
• W2032014069 countsByYear W20320140692017 @default.
• W2032014069 countsByYear W20320140692018 @default.
• W2032014069 countsByYear W20320140692021 @default.
• W2032014069 crossrefType "journal-article" @default.
• W2032014069 hasAuthorship W2032014069A5007107739 @default.
• W2032014069 hasAuthorship W2032014069A5022331909 @default.
• W2032014069 hasAuthorship W2032014069A5054219492 @default.
• W2032014069 hasAuthorship W2032014069A5072284526 @default.
• W2032014069 hasAuthorship W2032014069A5076718607 @default.
• W2032014069 hasAuthorship W2032014069A5084187130 @default.
• W2032014069 hasBestOaLocation W20320140691 @default.
• W2032014069 hasConcept C134088382 @default.
• W2032014069 hasConcept C135285700 @default.
• W2032014069 hasConcept C139407321 @default.
• W2032014069 hasConcept C144647389 @default.
• W2032014069 hasConcept C146727910 @default.
• W2032014069 hasConcept C163181125 @default.
• W2032014069 hasConcept C181199279 @default.
• W2032014069 hasConcept C185592680 @default.
• W2032014069 hasConcept C2777993845 @default.
• W2032014069 hasConcept C2779201268 @default.
• W2032014069 hasConcept C41625074 @default.
• W2032014069 hasConcept C55493867 @default.
• W2032014069 hasConcept C62478195 @default.
• W2032014069 hasConcept C79266657 @default.
• W2032014069 hasConcept C80631254 @default.
• W2032014069 hasConcept C86803240 @default.
• W2032014069 hasConcept C95444343 @default.
• W2032014069 hasConceptScore W2032014069C134088382 @default.
• W2032014069 hasConceptScore W2032014069C135285700 @default.
• W2032014069 hasConceptScore W2032014069C139407321 @default.
• W2032014069 hasConceptScore W2032014069C144647389 @default.
• W2032014069 hasConceptScore W2032014069C146727910 @default.
• W2032014069 hasConceptScore W2032014069C163181125 @default.
• W2032014069 hasConceptScore W2032014069C181199279 @default.
• W2032014069 hasConceptScore W2032014069C185592680 @default.
• W2032014069 hasConceptScore W2032014069C2777993845 @default.
• W2032014069 hasConceptScore W2032014069C2779201268 @default.
• W2032014069 hasConceptScore W2032014069C41625074 @default.
• W2032014069 hasConceptScore W2032014069C55493867 @default.
• W2032014069 hasConceptScore W2032014069C62478195 @default.

• next