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- W2032039046 abstract "Summary The apicoplast of Plasmodium falciparum carries a 35 kb circular genome (plDNA) that replicates at the late trophozoite stage of the parasite intraerythocytic cycle. plDNA replication proceeds predominantly via a d ‐loop/bi‐directional ori mechanism with replication ori localized within inverted repeat region. Although replication of the apicoplast genome is a validated drug target, the proteins involved in the replication process are only partially characterized. We analysed DNA–protein interactions at a plDNA replication ori region and report the identification of a nuclear‐encoded DnaJ homologue that binds directly to ori elements of the plDNA molecule. PfDnaJ A interacted with the minor groove of the DNA double‐helix and recognized a 13 bp sequence within the ori . Inhibition of binding with anti‐PfDnaJ A antibodies confirmed identity of the protein in DNA‐binding experiments with organellar protein fractions. The DNA‐binding domain of the ∼69 kDa PfDnaJ A lay within the N‐terminal 38 kDa region that carries DnaJ signature motifs. In contrast to PfDnaJ A in parasite organellar fractions, the recombinant protein interacted with DNA in a sequence non‐specific manner. Our results suggest a role for PfDnaJ A in replication/repair of the apicoplast genome." @default.
- W2032039046 created "2016-06-24" @default.
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- W2032039046 date "2010-02-01" @default.
- W2032039046 modified "2023-10-16" @default.
- W2032039046 title "Nuclear-encoded DnaJ homologue of<i>Plasmodium falciparum</i>interacts with replication<i>ori</i>of the apicoplast genome" @default.
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- W2032039046 doi "https://doi.org/10.1111/j.1365-2958.2009.07033.x" @default.
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