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- W2032040905 startingPage "549" @default.
- W2032040905 abstract "Specific protein domains known as protein transduction domains (PTDs) can permeate cell membranes and deliver proteins or bioactive materials into living cells. Various approaches have been applied for improving their transduction efficacy. It is, therefore, crucial to clarify the entry mechanisms and to identify the rate-limiting steps. Because of technical limitations for imaging PTD behavior on cells with conventional fluorescent-dyes, how PTDs enter the cells has been a topic of much debate. Utilizing quantum dots (QDs), we recently tracked the behavior of PTD that was derived from HIV-1 Tat (TatP) in living cells at the single-molecule level with 7-nm special precision. In this review article, we initially summarize the controversy on TatP entry mechanisms; thereafter, we will focus on our recent findings on single-TatP-QD tracking (SQT), to identify the major sequential steps of intracellular delivery in living cells and to discuss how SQT can easily provide direct information on TatP entry mechanisms. As a primer for SQT study, we also discuss the latest findings on single particle tracking of various molecules on the plasma membrane. Finally, we discuss the problems of QDs and the challenges for the future in utilizing currently available QD probes for SQT. In conclusion, direct identification of the rate-limiting steps of PTD entry with SQT should dramatically improve the methods for enhancing transduction efficiency." @default.
- W2032040905 created "2016-06-24" @default.
- W2032040905 creator A5073588329 @default.
- W2032040905 date "2012-01-05" @default.
- W2032040905 modified "2023-09-24" @default.
- W2032040905 title "Exploring Transduction Mechanisms of Protein Transduction Domains (PTDs) in Living Cells Utilizing Single-Quantum Dot Tracking (SQT) Technology" @default.
- W2032040905 cites W1488249325 @default.
- W2032040905 cites W1510613202 @default.
- W2032040905 cites W1616350046 @default.
- W2032040905 cites W1963768207 @default.
- W2032040905 cites W1965913747 @default.
- W2032040905 cites W1965964845 @default.
- W2032040905 cites W1969635978 @default.
- W2032040905 cites W1971217997 @default.
- W2032040905 cites W1973920331 @default.
- W2032040905 cites W1976822357 @default.
- W2032040905 cites W1984911127 @default.
- W2032040905 cites W1985222229 @default.
- W2032040905 cites W1986490461 @default.
- W2032040905 cites W1987854928 @default.
- W2032040905 cites W1989019324 @default.
- W2032040905 cites W1992095148 @default.
- W2032040905 cites W1992230504 @default.
- W2032040905 cites W1994914276 @default.
- W2032040905 cites W1995256659 @default.
- W2032040905 cites W1996346894 @default.
- W2032040905 cites W1996685148 @default.
- W2032040905 cites W1998820229 @default.
- W2032040905 cites W1998948607 @default.
- W2032040905 cites W2001582257 @default.
- W2032040905 cites W2003658370 @default.
- W2032040905 cites W2004927584 @default.
- W2032040905 cites W2008594150 @default.
- W2032040905 cites W2008813620 @default.
- W2032040905 cites W2010659535 @default.
- W2032040905 cites W2015404233 @default.
- W2032040905 cites W2016184924 @default.
- W2032040905 cites W2019144805 @default.
- W2032040905 cites W2019563202 @default.
- W2032040905 cites W2023612132 @default.
- W2032040905 cites W2028138612 @default.
- W2032040905 cites W2028567429 @default.
- W2032040905 cites W2028890857 @default.
- W2032040905 cites W2029346749 @default.
- W2032040905 cites W2031715022 @default.
- W2032040905 cites W2033001450 @default.
- W2032040905 cites W2033782734 @default.
- W2032040905 cites W2041922085 @default.
- W2032040905 cites W2042143995 @default.
- W2032040905 cites W2044161294 @default.
- W2032040905 cites W2055296721 @default.
- W2032040905 cites W2056329949 @default.
- W2032040905 cites W205758415 @default.
- W2032040905 cites W2057973294 @default.
- W2032040905 cites W2061120502 @default.
- W2032040905 cites W2062485439 @default.
- W2032040905 cites W2064287955 @default.
- W2032040905 cites W2067592236 @default.
- W2032040905 cites W2070554638 @default.
- W2032040905 cites W2071317718 @default.
- W2032040905 cites W2072113905 @default.
- W2032040905 cites W2076859770 @default.
- W2032040905 cites W2077337705 @default.
- W2032040905 cites W2079335891 @default.
- W2032040905 cites W2079981758 @default.
- W2032040905 cites W2081545569 @default.
- W2032040905 cites W2084729729 @default.
- W2032040905 cites W2085796374 @default.
- W2032040905 cites W2087419277 @default.
- W2032040905 cites W2088960285 @default.
- W2032040905 cites W2091000092 @default.
- W2032040905 cites W2091181345 @default.
- W2032040905 cites W2091204518 @default.
- W2032040905 cites W2103262726 @default.
- W2032040905 cites W2105668301 @default.
- W2032040905 cites W2108055113 @default.
- W2032040905 cites W2114656957 @default.
- W2032040905 cites W2123650819 @default.
- W2032040905 cites W2125449455 @default.
- W2032040905 cites W2132759053 @default.
- W2032040905 cites W2137210959 @default.
- W2032040905 cites W2139740129 @default.
- W2032040905 cites W2143168912 @default.
- W2032040905 cites W2146144253 @default.
- W2032040905 cites W2146255300 @default.
- W2032040905 cites W2147688960 @default.
- W2032040905 cites W2147845309 @default.
- W2032040905 cites W2148924929 @default.
- W2032040905 cites W2154321074 @default.
- W2032040905 cites W2154827556 @default.
- W2032040905 cites W2160863389 @default.
- W2032040905 cites W2164565944 @default.
- W2032040905 cites W2169594496 @default.
- W2032040905 cites W2170932434 @default.
- W2032040905 cites W2319708251 @default.
- W2032040905 cites W4238813159 @default.
- W2032040905 cites W4239028853 @default.
- W2032040905 doi "https://doi.org/10.3390/s120100549" @default.