FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032049863> ?p ?o ?g. }

• W2032049863 endingPage "3244" @default.
• W2032049863 startingPage "3235" @default.
• W2032049863 abstract "Genomic methylation patterns originate during gametogenesis and are postulated to be involved in important developmental events, including gene regulation, embryogenesis, and genomic imprinting. In previous work, treatment of male rats with 5-azacytidine, a drug that blocks DNA methylation, resulted in abnormal embryo development when germ cells were exposed throughout spermatogenesis, encompassing mitotic, meiotic, and postmeiotic development, but not if they were only exposed postmeiotically. To explore the mechanisms underlying the effects of 5-azacytidine on sperm function, we determined the effects of the drug on testicular morphology, assessed whether exposure of meiotic spermatocytes resulted in abnormal pregnancy outcome, and examined the role of germ cell genomic demethylation in mediating the effects of 5-azacytidine on spermatogonia and spermatocytes. Male Sprague Dawley rats were treated three times a week with saline or 5-azacytidine (2.5 and 4.0 mg/kg) for 6 weeks (meiotic and postmeiotic germ cell exposure) and 11 weeks (mitotic, meiotic, and postmeiotic exposure). Six weeks of paternal treatment with the highest dose of 5-azacytidine resulted in an increase in preimplantation loss (corpora lutea minus implantation sites) without affecting testicular morphology or altering sperm DNA methylation levels. Eleven weeks of 5-azacytidine treatment at doses that cause preimplantation loss resulted in severe abnormalities of the seminiferous tubules, such as degeneration and loss of germ cells, atrophy of seminiferous tubules, presence of multinuclear giant cells, and sloughing of immature germ cells into the lumen, and a 22–29% decrease in genomic methylation levels in epididymal sperm. On closer evaluation of testicular histology using terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end-labeling detection in situ, both 6 and 11 weeks of 5-azacytidine treatment resulted in an increase over the control value in the number of apoptotic germ cells in the seminiferous tubules. Analysis of DNA methylation levels in isolated germ cells of treated males indicated that spermatogonia were more susceptible to the hypomethylating effects of 5-azacytidine than were spermatocytes. These studies provide evidence of an association between demethylation of germ cell DNA and alterations in testicular histology." @default.
• W2032049863 created "2016-06-24" @default.
• W2032049863 creator A5047517794 @default.
• W2032049863 creator A5063193854 @default.
• W2032049863 creator A5071283358 @default.
• W2032049863 date "2000-09-01" @default.
• W2032049863 modified "2023-10-16" @default.
• W2032049863 title "Deoxyribonucleic Acid Hypomethylation of Male Germ Cells by Mitotic and Meiotic Exposure to 5-Azacytidine Is Associated with Altered Testicular Histology*" @default.
• W2032049863 cites W142190260 @default.
• W2032049863 cites W1572264474 @default.
• W2032049863 cites W1831299015 @default.
• W2032049863 cites W1923248627 @default.
• W2032049863 cites W1929982226 @default.
• W2032049863 cites W1976208541 @default.
• W2032049863 cites W1979273476 @default.
• W2032049863 cites W1993858796 @default.
• W2032049863 cites W1996501957 @default.
• W2032049863 cites W1996520212 @default.
• W2032049863 cites W1997921267 @default.
• W2032049863 cites W2016759352 @default.
• W2032049863 cites W2024088186 @default.
• W2032049863 cites W2025911406 @default.
• W2032049863 cites W2029892426 @default.
• W2032049863 cites W2031406286 @default.
• W2032049863 cites W2036300762 @default.
• W2032049863 cites W2042846772 @default.
• W2032049863 cites W2042938013 @default.
• W2032049863 cites W2046241907 @default.
• W2032049863 cites W2055171335 @default.
• W2032049863 cites W2055458870 @default.
• W2032049863 cites W2056527769 @default.
• W2032049863 cites W2056748053 @default.
• W2032049863 cites W2072181069 @default.
• W2032049863 cites W2074199894 @default.
• W2032049863 cites W2080051104 @default.
• W2032049863 cites W2084101481 @default.
• W2032049863 cites W2097151066 @default.
• W2032049863 cites W2104043690 @default.
• W2032049863 cites W2107976115 @default.
• W2032049863 cites W2120906967 @default.
• W2032049863 cites W2129461500 @default.
• W2032049863 cites W2144514579 @default.
• W2032049863 cites W2145750252 @default.
• W2032049863 cites W2146141565 @default.
• W2032049863 cites W2149350777 @default.
• W2032049863 cites W2154055274 @default.
• W2032049863 cites W2157896232 @default.
• W2032049863 cites W2165402393 @default.
• W2032049863 cites W2175849508 @default.
• W2032049863 cites W2182746672 @default.
• W2032049863 cites W2252403865 @default.
• W2032049863 cites W2322667628 @default.
• W2032049863 cites W4250662177 @default.
• W2032049863 doi "https://doi.org/10.1210/endo.141.9.7661" @default.
• W2032049863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10965894" @default.
• W2032049863 hasPublicationYear "2000" @default.
• W2032049863 type Work @default.
• W2032049863 sameAs 2032049863 @default.
• W2032049863 citedByCount "51" @default.
• W2032049863 countsByYear W20320498632012 @default.
• W2032049863 countsByYear W20320498632014 @default.
• W2032049863 countsByYear W20320498632015 @default.
• W2032049863 countsByYear W20320498632016 @default.
• W2032049863 countsByYear W20320498632017 @default.
• W2032049863 countsByYear W20320498632019 @default.
• W2032049863 countsByYear W20320498632020 @default.
• W2032049863 countsByYear W20320498632021 @default.
• W2032049863 countsByYear W20320498632022 @default.
• W2032049863 crossrefType "journal-article" @default.
• W2032049863 hasAuthorship W2032049863A5047517794 @default.
• W2032049863 hasAuthorship W2032049863A5063193854 @default.
• W2032049863 hasAuthorship W2032049863A5071283358 @default.
• W2032049863 hasBestOaLocation W20320498631 @default.
• W2032049863 hasConcept C104317684 @default.
• W2032049863 hasConcept C123765429 @default.
• W2032049863 hasConcept C126322002 @default.
• W2032049863 hasConcept C134018914 @default.
• W2032049863 hasConcept C148196450 @default.
• W2032049863 hasConcept C150194340 @default.
• W2032049863 hasConcept C16685009 @default.
• W2032049863 hasConcept C190727270 @default.
• W2032049863 hasConcept C196843134 @default.
• W2032049863 hasConcept C201492766 @default.
• W2032049863 hasConcept C2779122487 @default.
• W2032049863 hasConcept C2781087480 @default.
• W2032049863 hasConcept C39297687 @default.
• W2032049863 hasConcept C54355233 @default.
• W2032049863 hasConcept C71924100 @default.
• W2032049863 hasConcept C86803240 @default.
• W2032049863 hasConcept C87073359 @default.
• W2032049863 hasConceptScore W2032049863C104317684 @default.
• W2032049863 hasConceptScore W2032049863C123765429 @default.
• W2032049863 hasConceptScore W2032049863C126322002 @default.
• W2032049863 hasConceptScore W2032049863C134018914 @default.
• W2032049863 hasConceptScore W2032049863C148196450 @default.
• W2032049863 hasConceptScore W2032049863C150194340 @default.
• W2032049863 hasConceptScore W2032049863C16685009 @default.
• W2032049863 hasConceptScore W2032049863C190727270 @default.

• next