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- W2032056102 abstract "Freshly isolated hepatocytes, endothelial cells, Kupffer and fat-storing cells from adult rats were found to be able to form spheroidal aggregates within 24 hr when cultured under serum-free and rotatory conditions. The ultrastructure, including intracellular organization and extracellular matrix deposition was investigated in 7-day aggregates by scanning and electron microscopy. The different cell types expressed a histotypic organization and reconstructed their extracellular matrix (fibronectin and laminin) as well as junctional complexes (desmosomes, tight junctions and gap junctions). The aggregates preserved, over a period of at least 7 days, high albumin expression (mRNA) and secretion as well as high secretion of the acute phase protein T-kininogen. Dexamethasone (10−7 M) increased the tyrosine aminotransferase activity fourfold after a 24-hr exposure. Aggregates cultured in the presence of 10−7 M dexamethasone showed an increased expression and secretion of albumin concomitantly with a decrease in T-kininogen secretion. Induction of 7-ethoxycoumarin O-deethylase (ECOD) was effective after exposure to 3-methylcholanthrene or Aroclor for 48 hr. Up to a 13-fold increase in (ECOD) activity was found. The results demonstrate that spheroidal cultures of liver cell aggregates obtained under rotatory conditions provide a useful model for studies on the combined cellular interactions between non-parenchymal liver cells and hepatocytes from adult rats." @default.
- W2032056102 created "2016-06-24" @default.
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- W2032056102 date "1997-02-01" @default.
- W2032056102 modified "2023-09-29" @default.
- W2032056102 title "Expression of organ-specific structures and functions in long-term cultures of aggregates from adult rat liver cells" @default.
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- W2032056102 doi "https://doi.org/10.1016/s0887-2333(96)00065-3" @default.
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