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- W2032058119 abstract "Abstract Mice fed a combination of 10 per cent (v/v) ethanol and 5 per cent (w/v) sucrose for 5 months showed a 2-fold increase in the amount of [5- 3 H]uridine label that was incorporated into putative mRNA in the polysomes of the liver. Part of the increase was due to a change in the total amount of UTP present in the cell, although the uptake and processing of [5- 3 H]UTP were not altered. When corrected for UTP pool size variation, ethanol-sucrose-treated mice still incorporated 1.5-fold more [5- 3 H]uridine-labeled RNA into polysomes than controls. Poly(A + )mRNA content was 1.2 times greater in ethanol-sucrose-treated mice, and [5- 3 H]poly(U) hybridization t poly(A) tracts may be 1.5 times longer in ethanol-sucrose-treated mice than in controls. [Poly(A)mRNA is the same as poly(A + )mRNA]. The proportion of newly labeled poly(A − )mRNA declined in ethanol-sucrose-treated mice compared to controls. The results indicate that ethanol-sucrose treatment may cause an increase in the amount of poly(A + )mRNA that has longer poly(A) tracts and a concomitant decline in the amount of [5- 3 H]uridine label incorporated into poly(A − )mRNA. A decline in protein synthesis could not be explained by a lack of mRNA in ethanol-sucrose fed mice." @default.
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- W2032058119 date "1980-11-01" @default.
- W2032058119 modified "2023-09-27" @default.
- W2032058119 title "Effect of chronic ethanol and sucrose ingestion on liver polysomal poly(A)mRNA content and incorporation of [5-3H]uridine into mRNA" @default.
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- W2032058119 doi "https://doi.org/10.1016/0006-2952(80)90049-0" @default.
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