FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032079164> ?p ?o ?g. }

• W2032079164 endingPage "171" @default.
• W2032079164 startingPage "159" @default.
• W2032079164 abstract "Spinal cord injury in adult mammals causes atrophy or death of some axotomized neurons. The product of the antiapoptotic gene Bcl-2 prevents neuron death in vivo. We delivered Bcl-2 by intraspinal injection of a DNA plasmid encoding this gene to determine if axotomized neurons destined to undergo retrograde death could be rescued. Axons of the right side Clarke's Nucleus (CN) were cut unilaterally in adult Sprague-Dawley rats by T8 hemisection, leaving the contralateral (left) CN as an intact control. Two months postoperatively, there was ∼35% loss of total CN neurons in the right L1 segment. Only 15% of large CN neurons (>400 μm2), whose axons project to the cerebellum, survived—indicating atrophy and/or death of 85% of these cells. We injected a DNA plasmid encoding the human Bcl-2 gene and the bacterial reporter gene LacZ, which was complexed with cationic lipids, into the right side of segment T8 of the normal spinal cord, or just caudal to the hemisection site. The reporter gene was expressed in the perikarya of right CN neurons at L1 for up to 7 days, but not 14 days. Two months following T8 hemisection and Bcl-2/LacZ DNA injection, there was no significant loss of CN neurons ipsilateral to the lesion. Surprisingly, 61% of large neurons survived, indicating partial protection from atrophy. In contrast, a DNA plasmid that codes for the LacZ reporter gene, but not Bcl-2, did not prevent CN neuron death or atrophy. Administration of the Bcl-2 gene in adult rats and its expression in these CNS neurons prevents retrograde cell death, and also minimizes atrophy. These results may serve as the basis for developing novel gene therapy strategies for patients with spinal cord injury. J. Comp. Neurol. 404:159–171, 1999. © 1999 Wiley-Liss, Inc." @default.
• W2032079164 created "2016-06-24" @default.
• W2032079164 creator A5005813925 @default.
• W2032079164 creator A5022744507 @default.
• W2032079164 creator A5028159689 @default.
• W2032079164 creator A5064377500 @default.
• W2032079164 creator A5067718061 @default.
• W2032079164 creator A5079863884 @default.
• W2032079164 date "1999-02-08" @default.
• W2032079164 modified "2023-10-14" @default.
• W2032079164 title "DNA plasmid that codes for human Bcl-2 gene preserves axotomized Clarke's nucleus neurons and reduces atrophy after spinal cord hemisection in adult rats" @default.
• W2032079164 cites W107210068 @default.
• W2032079164 cites W1529927626 @default.
• W2032079164 cites W1549546756 @default.
• W2032079164 cites W1583699416 @default.
• W2032079164 cites W1657827244 @default.
• W2032079164 cites W1963523765 @default.
• W2032079164 cites W1964617786 @default.
• W2032079164 cites W1965169108 @default.
• W2032079164 cites W1975970556 @default.
• W2032079164 cites W1976857475 @default.
• W2032079164 cites W1979829045 @default.
• W2032079164 cites W1979850552 @default.
• W2032079164 cites W1982598770 @default.
• W2032079164 cites W1995261715 @default.
• W2032079164 cites W1997208169 @default.
• W2032079164 cites W2002778467 @default.
• W2032079164 cites W2004487226 @default.
• W2032079164 cites W2010610289 @default.
• W2032079164 cites W2017434751 @default.
• W2032079164 cites W2018068734 @default.
• W2032079164 cites W2022894945 @default.
• W2032079164 cites W2023964776 @default.
• W2032079164 cites W2027139822 @default.
• W2032079164 cites W2032333879 @default.
• W2032079164 cites W2033828028 @default.
• W2032079164 cites W2061497413 @default.
• W2032079164 cites W2066961524 @default.
• W2032079164 cites W2068796206 @default.
• W2032079164 cites W2077669223 @default.
• W2032079164 cites W2089519595 @default.
• W2032079164 cites W2108257172 @default.
• W2032079164 cites W2113999599 @default.
• W2032079164 cites W2126511939 @default.
• W2032079164 cites W2126706634 @default.
• W2032079164 cites W2144701362 @default.
• W2032079164 cites W2165836217 @default.
• W2032079164 cites W2166654160 @default.
• W2032079164 cites W4255244423 @default.
• W2032079164 cites W4298361955 @default.
• W2032079164 cites W48059278 @default.
• W2032079164 cites W86605765 @default.
• W2032079164 doi "https://doi.org/10.1002/(sici)1096-9861(19990208)404:2<159::aid-cne2>3.0.co;2-e" @default.
• W2032079164 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9934991" @default.
• W2032079164 hasPublicationYear "1999" @default.
• W2032079164 type Work @default.
• W2032079164 sameAs 2032079164 @default.
• W2032079164 citedByCount "43" @default.
• W2032079164 countsByYear W20320791642012 @default.
• W2032079164 countsByYear W20320791642013 @default.
• W2032079164 countsByYear W20320791642014 @default.
• W2032079164 countsByYear W20320791642015 @default.
• W2032079164 countsByYear W20320791642017 @default.
• W2032079164 countsByYear W20320791642019 @default.
• W2032079164 crossrefType "journal-article" @default.
• W2032079164 hasAuthorship W2032079164A5005813925 @default.
• W2032079164 hasAuthorship W2032079164A5022744507 @default.
• W2032079164 hasAuthorship W2032079164A5028159689 @default.
• W2032079164 hasAuthorship W2032079164A5064377500 @default.
• W2032079164 hasAuthorship W2032079164A5067718061 @default.
• W2032079164 hasAuthorship W2032079164A5079863884 @default.
• W2032079164 hasConcept C104317684 @default.
• W2032079164 hasConcept C150194340 @default.
• W2032079164 hasConcept C153911025 @default.
• W2032079164 hasConcept C161733203 @default.
• W2032079164 hasConcept C169760540 @default.
• W2032079164 hasConcept C190283241 @default.
• W2032079164 hasConcept C2778794669 @default.
• W2032079164 hasConcept C2780775167 @default.
• W2032079164 hasConcept C2781172350 @default.
• W2032079164 hasConcept C31573885 @default.
• W2032079164 hasConcept C54355233 @default.
• W2032079164 hasConcept C86803240 @default.
• W2032079164 hasConcept C95444343 @default.
• W2032079164 hasConceptScore W2032079164C104317684 @default.
• W2032079164 hasConceptScore W2032079164C150194340 @default.
• W2032079164 hasConceptScore W2032079164C153911025 @default.
• W2032079164 hasConceptScore W2032079164C161733203 @default.
• W2032079164 hasConceptScore W2032079164C169760540 @default.
• W2032079164 hasConceptScore W2032079164C190283241 @default.
• W2032079164 hasConceptScore W2032079164C2778794669 @default.
• W2032079164 hasConceptScore W2032079164C2780775167 @default.
• W2032079164 hasConceptScore W2032079164C2781172350 @default.
• W2032079164 hasConceptScore W2032079164C31573885 @default.
• W2032079164 hasConceptScore W2032079164C54355233 @default.
• W2032079164 hasConceptScore W2032079164C86803240 @default.
• W2032079164 hasConceptScore W2032079164C95444343 @default.
• W2032079164 hasIssue "2" @default.

• next