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• W2032126247 abstract "Abstract Co–B/SiO 2 /NH 2 magnetic nanoparticles (NPs) were prepared from a silica shell-coated Co–B core using the Stober method and amine-modification on the surface. Glucose oxidase (GOD) was covalently immobilized on the surface of Co–B/SiO 2 /NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The magnetic NPs characteristics, such as the synthesis of Co–B/SiO 2 /NH 2 NPs, effect of pH, temperature, and concentration of buffer for enzyme immobilization, were investigated. The optimal reaction conditions for immobilization were determined to be 0.1 M of phosphate buffer solution, pH 7.0, and 5 °C. In the case of immobilized GOD without d -glucose and with 0.1 M of d -glucose for blocking, 22.98 U/g and 24.83 U/g of their original activity were retained after 7 reuses, respectively." @default.
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• W2032126247 date "2012-08-01" @default.
• W2032126247 modified "2023-09-27" @default.
• W2032126247 title "Immobilization of glucose oxidase onto cobalt based on silica core/shell nanoparticles as carrier" @default.
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• W2032126247 doi "https://doi.org/10.1016/j.procbio.2012.04.027" @default.
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