FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032131490> ?p ?o ?g. }

• W2032131490 endingPage "467" @default.
• W2032131490 startingPage "455" @default.
• W2032131490 abstract "The seed oil of Anemone leveillei contains significant amounts of sciadonic acid (20:3Delta(5,11,14); SA), an unusual non-methylene-interrupted fatty acid with pharmaceutical potential similar to arachidonic acid. Two candidate cDNAs (AL10 and AL21) for the C(20) Delta(5cis)-desaturase from developing seeds of A. leveillei were functionally characterized in transgenic Arabidopsis (Arabidopsis thaliana) plants. The open reading frames of both Delta(5)-desaturases showed some similarity to presumptive acyl-coenzyme A (CoA) desaturases found in animals and plants. When expressed in transgenic Arabidopsis, AL21 showed a broad range of substrate specificity, utilizing both saturated (16:0 and 18:0) and unsaturated (18:2, n-6 and 18:3, n-3) substrates. In contrast, AL10 did not show any activity in wild-type Arabidopsis. Coexpression of AL10 or AL21 with a C(18) Delta(9)-elongase in transgenic Arabidopsis plants resulted in the production of SA and juniperonic fatty acid (20:4Delta(5,11,14,17)). Thus, AL10 acted only on C(20) polyunsaturated fatty acids in a manner analogous to front-end desaturases. However, neither AL10 nor AL21 contain the cytochrome b(5) domain normally present in this class of enzymes. Acyl-CoA profiling of transgenic Arabidopsis plants and developing A. leveillei seeds revealed significant accumulation of Delta(5)-unsaturated fatty acids as acyl-CoAs compared to the accumulation of these fatty acids in total lipids. Positional analysis of triacylglycerols of A. leveillei seeds showed that Delta(5)-desaturated fatty acids were present in both sn-2 and sn-1 + sn-3 positions, although the majority of 16:1Delta(5), 18:1Delta(5), and SA was present at the sn-2 position. Our data provide biochemical evidence for the A. leveillei Delta(5)-desaturases using acyl-CoA substrates." @default.
• W2032131490 created "2016-06-24" @default.
• W2032131490 creator A5000152115 @default.
• W2032131490 creator A5032872461 @default.
• W2032131490 creator A5073333495 @default.
• W2032131490 creator A5091791417 @default.
• W2032131490 date "2007-03-23" @default.
• W2032131490 modified "2023-10-18" @default.
• W2032131490 title "Cloning and Characterization of Unusual Fatty Acid Desaturases from <i>Anemone leveillei</i>: Identification of an Acyl-Coenzyme A C20 Δ5-Desaturase Responsible for the Synthesis of Sciadonic Acid" @default.
• W2032131490 cites W1605022478 @default.
• W2032131490 cites W1974442662 @default.
• W2032131490 cites W1987552897 @default.
• W2032131490 cites W1988973547 @default.
• W2032131490 cites W1996358681 @default.
• W2032131490 cites W1996588744 @default.
• W2032131490 cites W1998342535 @default.
• W2032131490 cites W1999268654 @default.
• W2032131490 cites W2009433062 @default.
• W2032131490 cites W2012906807 @default.
• W2032131490 cites W2015504740 @default.
• W2032131490 cites W2015978734 @default.
• W2032131490 cites W2024082478 @default.
• W2032131490 cites W2026103104 @default.
• W2032131490 cites W2026643194 @default.
• W2032131490 cites W2030830047 @default.
• W2032131490 cites W2032336298 @default.
• W2032131490 cites W2033475253 @default.
• W2032131490 cites W2033692422 @default.
• W2032131490 cites W2042830509 @default.
• W2032131490 cites W2044715107 @default.
• W2032131490 cites W2051850941 @default.
• W2032131490 cites W2059268226 @default.
• W2032131490 cites W2066011377 @default.
• W2032131490 cites W2069807866 @default.
• W2032131490 cites W2075981555 @default.
• W2032131490 cites W2076445462 @default.
• W2032131490 cites W2076584142 @default.
• W2032131490 cites W2077683858 @default.
• W2032131490 cites W2082546405 @default.
• W2032131490 cites W2083555012 @default.
• W2032131490 cites W2084322369 @default.
• W2032131490 cites W2086555622 @default.
• W2032131490 cites W2088655445 @default.
• W2032131490 cites W2090627940 @default.
• W2032131490 cites W2095221809 @default.
• W2032131490 cites W2100409339 @default.
• W2032131490 cites W2102716658 @default.
• W2032131490 cites W2110200485 @default.
• W2032131490 cites W2116122104 @default.
• W2032131490 cites W2119206918 @default.
• W2032131490 cites W2140385879 @default.
• W2032131490 cites W2154689388 @default.
• W2032131490 cites W2166213074 @default.
• W2032131490 cites W2323929242 @default.
• W2032131490 doi "https://doi.org/10.1104/pp.107.098202" @default.
• W2032131490 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1913799" @default.
• W2032131490 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17384161" @default.
• W2032131490 hasPublicationYear "2007" @default.
• W2032131490 type Work @default.
• W2032131490 sameAs 2032131490 @default.
• W2032131490 citedByCount "33" @default.
• W2032131490 countsByYear W20321314902012 @default.
• W2032131490 countsByYear W20321314902013 @default.
• W2032131490 countsByYear W20321314902015 @default.
• W2032131490 countsByYear W20321314902016 @default.
• W2032131490 countsByYear W20321314902017 @default.
• W2032131490 countsByYear W20321314902018 @default.
• W2032131490 countsByYear W20321314902020 @default.
• W2032131490 countsByYear W20321314902021 @default.
• W2032131490 countsByYear W20321314902022 @default.
• W2032131490 countsByYear W20321314902023 @default.
• W2032131490 crossrefType "journal-article" @default.
• W2032131490 hasAuthorship W2032131490A5000152115 @default.
• W2032131490 hasAuthorship W2032131490A5032872461 @default.
• W2032131490 hasAuthorship W2032131490A5073333495 @default.
• W2032131490 hasAuthorship W2032131490A5091791417 @default.
• W2032131490 hasBestOaLocation W20321314901 @default.
• W2032131490 hasConcept C104317684 @default.
• W2032131490 hasConcept C127078168 @default.
• W2032131490 hasConcept C134651460 @default.
• W2032131490 hasConcept C143065580 @default.
• W2032131490 hasConcept C181199279 @default.
• W2032131490 hasConcept C19038510 @default.
• W2032131490 hasConcept C2775988993 @default.
• W2032131490 hasConcept C2778078955 @default.
• W2032131490 hasConcept C2779123261 @default.
• W2032131490 hasConcept C2779491563 @default.
• W2032131490 hasConcept C2780809830 @default.
• W2032131490 hasConcept C543025807 @default.
• W2032131490 hasConcept C55493867 @default.
• W2032131490 hasConcept C86803240 @default.
• W2032131490 hasConceptScore W2032131490C104317684 @default.
• W2032131490 hasConceptScore W2032131490C127078168 @default.
• W2032131490 hasConceptScore W2032131490C134651460 @default.
• W2032131490 hasConceptScore W2032131490C143065580 @default.
• W2032131490 hasConceptScore W2032131490C181199279 @default.
• W2032131490 hasConceptScore W2032131490C19038510 @default.
• W2032131490 hasConceptScore W2032131490C2775988993 @default.

• next