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• W2032144485 abstract "R.MwoI, a type-II restriction enzyme with the new specificity 5'-GCN7GC-3', was found in extracts of the thermophilic archaebacterium, Methanobacterium wolfei. R.MwoI cleaves duplex DNA producing fragments with 3-nt, 3'-terminal extensions, thus: GCN5/N2GC. The genes coding for the MwoI restriction and modification enzymes were cloned into Escherichia coli on the plasmid vector pBR322. The clones synthesize a low level of R.MwoI endonuclease. The plasmids display incomplete MwoI-specific modification, suggesting that the clones synthesize a low level of the M.MwoI methyltransferase, too." @default.
• W2032144485 created "2016-06-24" @default.
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• W2032144485 date "1989-04-01" @default.
• W2032144485 modified "2023-09-27" @default.
• W2032144485 title "Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei" @default.
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• W2032144485 doi "https://doi.org/10.1016/0378-1119(89)90354-5" @default.
• W2032144485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2663652" @default.
• W2032144485 hasPublicationYear "1989" @default.
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