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- W2032177745 abstract "A new chemically defined medium consisting of equal parts of Dulbecco modified Eagle's and Ham's F-12 media supplemented with insulin, sodium selenite, putrescine, and D+ galactose, which allows the longterm survival of mature oligodendrocyte pure cultures, is described. Immunohistochemical staining has shown that over 90% of the cells become positive for myelin proteins shortly following subculture. Contaminating astrocytes (2%) do not survive in this medium. Biochemical data have indicated that these purified oligodendrocytes express 2′ 3′-cyclic nucleotide 3′-phosphohydrolase and UDP-galactose ceramide galactosyltransferase activities. Electoron microscopical examination revealed that the oligodendrocytes were mostly of medium-dark type and appeared to be identical to cells cultured in serum-containing medium. The ability to maintain pure oligodendrocyte cultures in such a defined medium will allow investigations concerning exogenous and endogenous factors involved in oligodendrocyte metabolism." @default.
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- W2032177745 date "1988-02-01" @default.
- W2032177745 modified "2023-10-18" @default.
- W2032177745 title "A chemically defined medium for the culture of mature oligodendrocytes" @default.
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- W2032177745 doi "https://doi.org/10.1002/jnr.490190205" @default.
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