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- W2032258002 abstract "Importin beta mediates active passage of cellular substrates through the nuclear pore complex (NPC). Adaptors such as importin alpha and snurportin associate with importin beta via an importin beta binding (IBB) domain. The intrinsic structural flexibility of importin beta allows its concerted interactions with IBB domains, phenylalanine-glycine nucleoporins, and the GTPase Ran during transport. In this paper, we provide evidence that the nature of the IBB domain modulates the affinity of the import complex for the NPC. In permeabilized cells, importin beta imports a cargo fused to the snurportin IBB (sIBB) with approximately 70% reduced energy requirement as compared with the classical importin alpha IBB. At the molecular level, this is explained by approximately 200-fold reduced affinity of importin beta for Nup62, when bound to the sIBB. Consistently, in vivo, the importin beta.sIBB complex has greatly reduced persistence inside the central channel of the NPC. We propose that by controlling the degree of strain in the tertiary structure of importin beta, the IBB domain modulates the affinity of the import complex for nucleoporins, thus dictating its persistence inside the NPC." @default.
- W2032258002 created "2016-06-24" @default.
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- W2032258002 date "2010-04-01" @default.
- W2032258002 modified "2023-10-02" @default.
- W2032258002 title "The Importin β Binding Domain Modulates the Avidity of Importin β for the Nuclear Pore Complex" @default.
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- W2032258002 doi "https://doi.org/10.1074/jbc.m109.095760" @default.
- W2032258002 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2859540" @default.
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