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- W2032330364 abstract "Raw sago starch digesting amylase was obtained from Penicillium brunneum No. 24. The crude enzyme from this strain contains carboxymethylcellulase (CMC-ase), avicelase, α-amylase and α-glucosidase. Affinity chromatography (α-cyclodextrin-Sepharose 6B) of the enzyme after ammonium sulfate fractionation, Toyopearl HW-55F gel filtration, DEAE-Sephadex A-50 and DEAE-cellulose chromatographies fractionation steps, resulted in a homogeneous glucoamylase. SDS-polyacrylamide gel electrophoresis of purified enzyme showed a single band, and a molecular weight of 80,000 for the native glucoamylase from Penicillium brunneum No. 24 was observed. After modification of the native glucoamylase with subtilisin, the molecular weight was reduced to 76,000. It lost the ability to digest and adsorb onto raw starches. However, its ability to digest gelatinized starches was preserved.Reinigung und Eigenschaften der Rohstarke abbauenden Amylase aus Penicillium brunneum Nr. 24.Aus Penicillium brunneum Nr. 24 wurde Rohstarke abbauende Amylase gewonnen. Das Rohenzym aus diesem Stamm enthalt Carboxymethylcellulase (CMC-ase), Avicelase, α-Amylase und α-Glucosidase. Die Affinitatschromatographie (α-Cyclodextrin-Sepharose 6B) des Enzyms nach Ammoniumsulfat-Fraktionierung, Toyopearl-HW-55F-Gelfiltration, DEAE-Sephadex-A-50- und DEAE-Cellulose-Chromatographie-Fraktionierungsstufen ergaben eine homogene Glucoamylase. Die SDS-Polyacrylamid-Gelelektrophorese zeigte ein einziges Band, und ein Molekulargewicht von 80.000 fur die native Glucoamylase aus Penicillium brunneum Nr. 24 wurde ermittelt. Nach Modifizierung der nativen Glucoamylase mit Subtilisin war das Molekulargewicht auf 76.000 reduziert. Es verlor die Fahigkeit des Abbaues und der Absorption an rohe Starken. Jedoch blieb seine Fahigkeit, verkleisterte Starken abzubauen, bestehen." @default.
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- W2032330364 date "1994-01-01" @default.
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- W2032330364 title "Purification and Properties of the Raw Starch Digesting Amylase fromPenicillium brunneum No. 24" @default.
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- W2032330364 doi "https://doi.org/10.1002/star.19940461207" @default.
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