FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032330926> ?p ?o ?g. }

• W2032330926 endingPage "72" @default.
• W2032330926 startingPage "64" @default.
• W2032330926 abstract "A novel β-glucosidase gene (designated PtBglu3) from Paecilomyces thermophila was cloned and sequenced. PtBglu3 has an open reading frame of 2,557 bp, encoding 858 amino acids with a calculated molecular mass of 90.9 kDa. The amino acid sequence of the mature polypeptide shared the highest identity (70%) to a glycoside hydrolase (GH) family 3 characterized β-glucosidase from Penicillium purpurogenum. PtBglu3 without the signal peptides was cloned into pPIC9K vector and successfully expressed in Pichia pastoris as an active extracellular β-glucosidase (PtBglu3). High activity of 274.4 U/ml was obtained by high cell-density fermentation, which is by far the highest reported yield for β-glucosidase. The recombinant enzyme was purified to homogeneity with 3.3-fold purification and a recovery of 68.5%. The molecular mass of the enzyme was estimated to be 116 kDa by SDS–PAGE, and 198.2 kDa by gel filtration, indicating that it was a dimer. Optimal activity of the purified enzyme was observed at pH 6.0 and 65 °C, and it was stable up to 60 °C. The enzyme exhibited high specific activity toward pNP-β-d-glucopyranoside, cellooligosaccharides, gentiobiose, amygdalin and salicin, and relatively lower activity against lichenan and laminarin. The present results should contribute to improving industrial production of β-glucosidase." @default.
• W2032330926 created "2016-06-24" @default.
• W2032330926 creator A5000093771 @default.
• W2032330926 creator A5009429704 @default.
• W2032330926 creator A5011696144 @default.
• W2032330926 creator A5047671159 @default.
• W2032330926 creator A5047980964 @default.
• W2032330926 date "2012-07-01" @default.
• W2032330926 modified "2023-09-27" @default.
• W2032330926 title "High level expression of extracellular secretion of a β-glucosidase gene (PtBglu3) from Paecilomyces thermophila in Pichia pastoris" @default.
• W2032330926 cites W1775749144 @default.
• W2032330926 cites W1830745998 @default.
• W2032330926 cites W1969192227 @default.
• W2032330926 cites W1970160066 @default.
• W2032330926 cites W1975132604 @default.
• W2032330926 cites W1981905690 @default.
• W2032330926 cites W1983994000 @default.
• W2032330926 cites W1991841130 @default.
• W2032330926 cites W1994908713 @default.
• W2032330926 cites W1999113569 @default.
• W2032330926 cites W2005133608 @default.
• W2032330926 cites W2020864718 @default.
• W2032330926 cites W2027487272 @default.
• W2032330926 cites W2036771706 @default.
• W2032330926 cites W2037305697 @default.
• W2032330926 cites W2051082836 @default.
• W2032330926 cites W2053038740 @default.
• W2032330926 cites W2055159539 @default.
• W2032330926 cites W2061185706 @default.
• W2032330926 cites W2067076463 @default.
• W2032330926 cites W2069879990 @default.
• W2032330926 cites W2077034564 @default.
• W2032330926 cites W2083381259 @default.
• W2032330926 cites W2086081407 @default.
• W2032330926 cites W2090359391 @default.
• W2032330926 cites W2092663969 @default.
• W2032330926 cites W2094244596 @default.
• W2032330926 cites W2100837269 @default.
• W2032330926 cites W2105481366 @default.
• W2032330926 cites W2108217561 @default.
• W2032330926 cites W2112723141 @default.
• W2032330926 cites W2125312027 @default.
• W2032330926 cites W2125811524 @default.
• W2032330926 cites W2139101347 @default.
• W2032330926 cites W2142321455 @default.
• W2032330926 cites W2152108974 @default.
• W2032330926 cites W4211016795 @default.
• W2032330926 cites W4230288102 @default.
• W2032330926 doi "https://doi.org/10.1016/j.pep.2012.04.016" @default.
• W2032330926 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22568931" @default.
• W2032330926 hasPublicationYear "2012" @default.
• W2032330926 type Work @default.
• W2032330926 sameAs 2032330926 @default.
• W2032330926 citedByCount "27" @default.
• W2032330926 countsByYear W20323309262012 @default.
• W2032330926 countsByYear W20323309262013 @default.
• W2032330926 countsByYear W20323309262014 @default.
• W2032330926 countsByYear W20323309262015 @default.
• W2032330926 countsByYear W20323309262017 @default.
• W2032330926 countsByYear W20323309262018 @default.
• W2032330926 countsByYear W20323309262019 @default.
• W2032330926 countsByYear W20323309262020 @default.
• W2032330926 countsByYear W20323309262021 @default.
• W2032330926 countsByYear W20323309262023 @default.
• W2032330926 crossrefType "journal-article" @default.
• W2032330926 hasAuthorship W2032330926A5000093771 @default.
• W2032330926 hasAuthorship W2032330926A5009429704 @default.
• W2032330926 hasAuthorship W2032330926A5011696144 @default.
• W2032330926 hasAuthorship W2032330926A5047671159 @default.
• W2032330926 hasAuthorship W2032330926A5047980964 @default.
• W2032330926 hasConcept C104317684 @default.
• W2032330926 hasConcept C153911025 @default.
• W2032330926 hasConcept C167625842 @default.
• W2032330926 hasConcept C181199279 @default.
• W2032330926 hasConcept C2779703480 @default.
• W2032330926 hasConcept C2780220376 @default.
• W2032330926 hasConcept C2781372317 @default.
• W2032330926 hasConcept C28212737 @default.
• W2032330926 hasConcept C40767141 @default.
• W2032330926 hasConcept C47289529 @default.
• W2032330926 hasConcept C515207424 @default.
• W2032330926 hasConcept C55493867 @default.
• W2032330926 hasConcept C58121356 @default.
• W2032330926 hasConcept C86803240 @default.
• W2032330926 hasConceptScore W2032330926C104317684 @default.
• W2032330926 hasConceptScore W2032330926C153911025 @default.
• W2032330926 hasConceptScore W2032330926C167625842 @default.
• W2032330926 hasConceptScore W2032330926C181199279 @default.
• W2032330926 hasConceptScore W2032330926C2779703480 @default.
• W2032330926 hasConceptScore W2032330926C2780220376 @default.
• W2032330926 hasConceptScore W2032330926C2781372317 @default.
• W2032330926 hasConceptScore W2032330926C28212737 @default.
• W2032330926 hasConceptScore W2032330926C40767141 @default.
• W2032330926 hasConceptScore W2032330926C47289529 @default.
• W2032330926 hasConceptScore W2032330926C515207424 @default.
• W2032330926 hasConceptScore W2032330926C55493867 @default.
• W2032330926 hasConceptScore W2032330926C58121356 @default.
• W2032330926 hasConceptScore W2032330926C86803240 @default.

• next