Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032474842> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2032474842 endingPage "208" @default.
- W2032474842 startingPage "201" @default.
- W2032474842 abstract "Isolated superior cervical ganglion (SCG) neurons were maintained in vitro at 22 degrees C (22 degrees C neurons) for up to 4 days in an effort to inhibit process outgrowth and thus extend the useful life of SCG neurons for voltage-clamp experiments. The neurons were viable after 4 days in vitro and remained roughly spherical whereas neurons maintained in vitro at 37 degrees C (37 degrees C neurons) developed extensive neurite processes after 2 days. The resting potential of 22 degrees C neurons was more hyperpolarized and the action potential duration was reduced compared to acutely isolated neurons (acute neurons) or 37 degrees C neurons. The steady state Na+ current activation and inactivation parameters of the acute and 22 degrees C neurons were similar whereas the half activation voltage was hyperpolarized and the slope factor of inactivation was reduced for the 37 degrees C neurons compared to the other two groups. The Na+ currents recorded from the 37 degrees C neurons displayed obvious signs of poor voltage control which were not observed in the acute or 22 degrees C neurons. The acetylcholine (ACh) sensitivity of both 22 degrees C and 37 degrees C neurons was significantly less than that of the acute neurons. This report demonstrates that room temperature culture of SCG neurons is a simple method which prevents process outgrowth and thus extends the useful life of the neurons for voltage-clamp experiments." @default.
- W2032474842 created "2016-06-24" @default.
- W2032474842 creator A5043288520 @default.
- W2032474842 creator A5057308878 @default.
- W2032474842 date "1991-07-01" @default.
- W2032474842 modified "2023-09-27" @default.
- W2032474842 title "Room temperature culture extends the useful life of adult neurons for voltage-clamp experiments" @default.
- W2032474842 cites W1603186852 @default.
- W2032474842 cites W1670400951 @default.
- W2032474842 cites W1972046379 @default.
- W2032474842 cites W1997555787 @default.
- W2032474842 cites W2009667219 @default.
- W2032474842 cites W2017656839 @default.
- W2032474842 cites W2022518699 @default.
- W2032474842 cites W2035149901 @default.
- W2032474842 cites W2056081019 @default.
- W2032474842 cites W2059363058 @default.
- W2032474842 cites W2060765203 @default.
- W2032474842 cites W2070241223 @default.
- W2032474842 cites W2082178747 @default.
- W2032474842 cites W2084544813 @default.
- W2032474842 cites W2087216042 @default.
- W2032474842 cites W2122066603 @default.
- W2032474842 cites W2130349600 @default.
- W2032474842 cites W2142117974 @default.
- W2032474842 cites W2252378079 @default.
- W2032474842 cites W2336721052 @default.
- W2032474842 doi "https://doi.org/10.1016/0165-0270(91)90170-5" @default.
- W2032474842 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1784123" @default.
- W2032474842 hasPublicationYear "1991" @default.
- W2032474842 type Work @default.
- W2032474842 sameAs 2032474842 @default.
- W2032474842 citedByCount "10" @default.
- W2032474842 countsByYear W20324748422016 @default.
- W2032474842 crossrefType "journal-article" @default.
- W2032474842 hasAuthorship W2032474842A5043288520 @default.
- W2032474842 hasAuthorship W2032474842A5057308878 @default.
- W2032474842 hasConcept C100837961 @default.
- W2032474842 hasConcept C105702510 @default.
- W2032474842 hasConcept C113246987 @default.
- W2032474842 hasConcept C12554922 @default.
- W2032474842 hasConcept C147944092 @default.
- W2032474842 hasConcept C169760540 @default.
- W2032474842 hasConcept C185263204 @default.
- W2032474842 hasConcept C185592680 @default.
- W2032474842 hasConcept C202751555 @default.
- W2032474842 hasConcept C2776161997 @default.
- W2032474842 hasConcept C2777151030 @default.
- W2032474842 hasConcept C2778794669 @default.
- W2032474842 hasConcept C2781142105 @default.
- W2032474842 hasConcept C2983650178 @default.
- W2032474842 hasConcept C31972630 @default.
- W2032474842 hasConcept C41008148 @default.
- W2032474842 hasConcept C55493867 @default.
- W2032474842 hasConcept C84111939 @default.
- W2032474842 hasConcept C86803240 @default.
- W2032474842 hasConceptScore W2032474842C100837961 @default.
- W2032474842 hasConceptScore W2032474842C105702510 @default.
- W2032474842 hasConceptScore W2032474842C113246987 @default.
- W2032474842 hasConceptScore W2032474842C12554922 @default.
- W2032474842 hasConceptScore W2032474842C147944092 @default.
- W2032474842 hasConceptScore W2032474842C169760540 @default.
- W2032474842 hasConceptScore W2032474842C185263204 @default.
- W2032474842 hasConceptScore W2032474842C185592680 @default.
- W2032474842 hasConceptScore W2032474842C202751555 @default.
- W2032474842 hasConceptScore W2032474842C2776161997 @default.
- W2032474842 hasConceptScore W2032474842C2777151030 @default.
- W2032474842 hasConceptScore W2032474842C2778794669 @default.
- W2032474842 hasConceptScore W2032474842C2781142105 @default.
- W2032474842 hasConceptScore W2032474842C2983650178 @default.
- W2032474842 hasConceptScore W2032474842C31972630 @default.
- W2032474842 hasConceptScore W2032474842C41008148 @default.
- W2032474842 hasConceptScore W2032474842C55493867 @default.
- W2032474842 hasConceptScore W2032474842C84111939 @default.
- W2032474842 hasConceptScore W2032474842C86803240 @default.
- W2032474842 hasIssue "2-3" @default.
- W2032474842 hasLocation W20324748421 @default.
- W2032474842 hasLocation W20324748422 @default.
- W2032474842 hasOpenAccess W2032474842 @default.
- W2032474842 hasPrimaryLocation W20324748421 @default.
- W2032474842 hasRelatedWork W2010421080 @default.
- W2032474842 hasRelatedWork W2022518699 @default.
- W2032474842 hasRelatedWork W2031906715 @default.
- W2032474842 hasRelatedWork W2032474842 @default.
- W2032474842 hasRelatedWork W2060407010 @default.
- W2032474842 hasRelatedWork W2138825478 @default.
- W2032474842 hasRelatedWork W2366444655 @default.
- W2032474842 hasRelatedWork W2434991433 @default.
- W2032474842 hasRelatedWork W3000457996 @default.
- W2032474842 hasRelatedWork W3083456528 @default.
- W2032474842 hasVolume "38" @default.
- W2032474842 isParatext "false" @default.
- W2032474842 isRetracted "false" @default.
- W2032474842 magId "2032474842" @default.
- W2032474842 workType "article" @default.