Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032508957> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2032508957 endingPage "278" @default.
- W2032508957 startingPage "266" @default.
- W2032508957 abstract "Photooxidation of chymotrypsin with visible light in the presence of traces of methylene blue resulted in rapid inactivation of the enzyme. Both the proteolytic and esterolytic activities were inactivated at the same rate. A 100 % inactivation of the enzyme took place at 4.0 moles of oxygen uptake per mole of enzyme, at which point 1 mole of histidine, of the total of 2 moles, and 3 moles of tryptophan, of the total of 7 moles, were photooxidized. Solubility and viscosity measurements indicated the absence of denaturation during the photooxidation of the enzyme. No hydrolysis of the peptide bonds of chymotrypsin could be observed during the photooxidation of this enzyme. Photooxidation of chymotrypsinogen to the extent of 4 moles of oxygen per mole of the precursor eliminated its ability to be converted into the active enzyme by traces of trypsin. Photo-inactivation of chymotrypsin eliminated the reactive site for the attachement of diisopropyl fluorophosphate. The possible significance of these findings in relation to the catalytic property of chymotrypsin is discussed." @default.
- W2032508957 created "2016-06-24" @default.
- W2032508957 creator A5029449680 @default.
- W2032508957 creator A5040800581 @default.
- W2032508957 creator A5050243504 @default.
- W2032508957 date "1953-10-01" @default.
- W2032508957 modified "2023-10-16" @default.
- W2032508957 title "Photooxidation of crystalline chymotrypsin in the presence of methylene blue" @default.
- W2032508957 cites W126813444 @default.
- W2032508957 cites W143058766 @default.
- W2032508957 cites W1572560039 @default.
- W2032508957 cites W1582102664 @default.
- W2032508957 cites W1612122265 @default.
- W2032508957 cites W16907096 @default.
- W2032508957 cites W1977513169 @default.
- W2032508957 cites W2043238197 @default.
- W2032508957 cites W2048499935 @default.
- W2032508957 cites W2069825226 @default.
- W2032508957 cites W2083198629 @default.
- W2032508957 cites W2086491465 @default.
- W2032508957 cites W2149417143 @default.
- W2032508957 cites W2170304969 @default.
- W2032508957 cites W2236082650 @default.
- W2032508957 cites W2243718301 @default.
- W2032508957 cites W2321411034 @default.
- W2032508957 cites W2328655867 @default.
- W2032508957 cites W2331235191 @default.
- W2032508957 cites W2332689691 @default.
- W2032508957 cites W2395246579 @default.
- W2032508957 cites W2395783725 @default.
- W2032508957 cites W2416464552 @default.
- W2032508957 cites W2417945660 @default.
- W2032508957 cites W2423204806 @default.
- W2032508957 doi "https://doi.org/10.1016/0003-9861(53)90200-8" @default.
- W2032508957 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/13092972" @default.
- W2032508957 hasPublicationYear "1953" @default.
- W2032508957 type Work @default.
- W2032508957 sameAs 2032508957 @default.
- W2032508957 citedByCount "179" @default.
- W2032508957 countsByYear W20325089572021 @default.
- W2032508957 crossrefType "journal-article" @default.
- W2032508957 hasAuthorship W2032508957A5029449680 @default.
- W2032508957 hasAuthorship W2032508957A5040800581 @default.
- W2032508957 hasAuthorship W2032508957A5050243504 @default.
- W2032508957 hasConcept C124815738 @default.
- W2032508957 hasConcept C13965031 @default.
- W2032508957 hasConcept C161790260 @default.
- W2032508957 hasConcept C178790620 @default.
- W2032508957 hasConcept C181199279 @default.
- W2032508957 hasConcept C185592680 @default.
- W2032508957 hasConcept C2776706248 @default.
- W2032508957 hasConcept C2776923230 @default.
- W2032508957 hasConcept C2778401398 @default.
- W2032508957 hasConcept C2778460671 @default.
- W2032508957 hasConcept C2779191827 @default.
- W2032508957 hasConcept C2780340462 @default.
- W2032508957 hasConcept C515207424 @default.
- W2032508957 hasConcept C55493867 @default.
- W2032508957 hasConcept C65165184 @default.
- W2032508957 hasConcept C75473681 @default.
- W2032508957 hasConcept C94412978 @default.
- W2032508957 hasConceptScore W2032508957C124815738 @default.
- W2032508957 hasConceptScore W2032508957C13965031 @default.
- W2032508957 hasConceptScore W2032508957C161790260 @default.
- W2032508957 hasConceptScore W2032508957C178790620 @default.
- W2032508957 hasConceptScore W2032508957C181199279 @default.
- W2032508957 hasConceptScore W2032508957C185592680 @default.
- W2032508957 hasConceptScore W2032508957C2776706248 @default.
- W2032508957 hasConceptScore W2032508957C2776923230 @default.
- W2032508957 hasConceptScore W2032508957C2778401398 @default.
- W2032508957 hasConceptScore W2032508957C2778460671 @default.
- W2032508957 hasConceptScore W2032508957C2779191827 @default.
- W2032508957 hasConceptScore W2032508957C2780340462 @default.
- W2032508957 hasConceptScore W2032508957C515207424 @default.
- W2032508957 hasConceptScore W2032508957C55493867 @default.
- W2032508957 hasConceptScore W2032508957C65165184 @default.
- W2032508957 hasConceptScore W2032508957C75473681 @default.
- W2032508957 hasConceptScore W2032508957C94412978 @default.
- W2032508957 hasIssue "2" @default.
- W2032508957 hasLocation W20325089571 @default.
- W2032508957 hasLocation W20325089572 @default.
- W2032508957 hasOpenAccess W2032508957 @default.
- W2032508957 hasPrimaryLocation W20325089571 @default.
- W2032508957 hasRelatedWork W1978497501 @default.
- W2032508957 hasRelatedWork W2048535205 @default.
- W2032508957 hasRelatedWork W2049444949 @default.
- W2032508957 hasRelatedWork W2053576006 @default.
- W2032508957 hasRelatedWork W2060034525 @default.
- W2032508957 hasRelatedWork W2060415280 @default.
- W2032508957 hasRelatedWork W2145515866 @default.
- W2032508957 hasRelatedWork W2159942393 @default.
- W2032508957 hasRelatedWork W2364515453 @default.
- W2032508957 hasRelatedWork W2376566455 @default.
- W2032508957 hasVolume "46" @default.
- W2032508957 isParatext "false" @default.
- W2032508957 isRetracted "false" @default.
- W2032508957 magId "2032508957" @default.
- W2032508957 workType "article" @default.