FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032533435> ?p ?o ?g. }

• W2032533435 endingPage "850" @default.
• W2032533435 startingPage "841" @default.
• W2032533435 abstract "The FixL protein is a heme-based sensor. Binding of oxygen to a unique heme domain inhibits a kinase domain of the type found in two-component regulators. Oxygen association is slow, but the dissociation rate is comparable to that of myoglobins. We have probed the size and chemistry of the FixL heme pocket by measuring the affinites, on rates and off rates for a wide variety of ferric heme ligands. Cyanide, but not fluoride, regulates the kinase activity. To examine how the sensory heme domain interacts with the kinase, we asked how the presence of the kinase domain affects ligand binding.The affinities of ferric FixL for heme ligands follow the same trend as their pKa values: cyanide > 4-methyl imidazole > imidazole > fluoride > azide >> thiocyanate. The association rates follow the reverse trend. Striking differences from myoglobin include a 6-fold greater affinity for, and faster binding to, the bulky ligand imidazole, a 14-fold faster on rate for nitric oxide, a 2 800-fold lower affinity for azide, and a complete failure to bind thiocyanate. The presence of the kinase domain does not alter the affinity or binding kinetics of the high-spin ligand fluoride, but affects the off rates of other ligands. The EPR spectrum shows a characteristic pentacoordinate nitrosyl heme, indicating that the Fe-His bond in FixL is strained.The importance of ligand deprotonation to the on rates and the fact that large ligands bind readily indicate that the heme pocket is open and apolar. Ligand basicity strongly influences the strength of binding. The destabilization of inhibitory ligands by the presence of the kinase domain is consistent with a 'load' imposed by coupling to the inactivating mechanism." @default.
• W2032533435 created "2016-06-24" @default.
• W2032533435 creator A5023133985 @default.
• W2032533435 creator A5029314765 @default.
• W2032533435 creator A5049448925 @default.
• W2032533435 creator A5050613763 @default.
• W2032533435 creator A5051198429 @default.
• W2032533435 creator A5060056480 @default.
• W2032533435 creator A5084922970 @default.
• W2032533435 creator A5089359104 @default.
• W2032533435 date "1996-10-01" @default.
• W2032533435 modified "2023-09-28" @default.
• W2032533435 title "Nonsteric factors dominate binding of nitric oxide, azide, imidazole, cyanide, and fluoride to the Rhizobial heme-based oxygen sensor FixL" @default.
• W2032533435 cites W1510941613 @default.
• W2032533435 cites W1514532383 @default.
• W2032533435 cites W1536460853 @default.
• W2032533435 cites W1538450226 @default.
• W2032533435 cites W1550187980 @default.
• W2032533435 cites W1589203341 @default.
• W2032533435 cites W1969723927 @default.
• W2032533435 cites W1973050146 @default.
• W2032533435 cites W1974936925 @default.
• W2032533435 cites W1993983571 @default.
• W2032533435 cites W2018778544 @default.
• W2032533435 cites W2020669931 @default.
• W2032533435 cites W2035562855 @default.
• W2032533435 cites W2036369693 @default.
• W2032533435 cites W2038691140 @default.
• W2032533435 cites W2044376403 @default.
• W2032533435 cites W2045563945 @default.
• W2032533435 cites W2050762712 @default.
• W2032533435 cites W2081725330 @default.
• W2032533435 cites W2086037622 @default.
• W2032533435 cites W2088616445 @default.
• W2032533435 cites W2096700300 @default.
• W2032533435 cites W2132397429 @default.
• W2032533435 cites W2410398939 @default.
• W2032533435 doi "https://doi.org/10.1016/s1074-5521(96)90070-8" @default.
• W2032533435 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8939703" @default.
• W2032533435 hasPublicationYear "1996" @default.
• W2032533435 type Work @default.
• W2032533435 sameAs 2032533435 @default.
• W2032533435 citedByCount "46" @default.
• W2032533435 countsByYear W20325334352012 @default.
• W2032533435 countsByYear W20325334352013 @default.
• W2032533435 countsByYear W20325334352014 @default.
• W2032533435 countsByYear W20325334352015 @default.
• W2032533435 countsByYear W20325334352016 @default.
• W2032533435 countsByYear W20325334352017 @default.
• W2032533435 countsByYear W20325334352018 @default.
• W2032533435 countsByYear W20325334352019 @default.
• W2032533435 countsByYear W20325334352021 @default.
• W2032533435 countsByYear W20325334352022 @default.
• W2032533435 countsByYear W20325334352023 @default.
• W2032533435 crossrefType "journal-article" @default.
• W2032533435 hasAuthorship W2032533435A5023133985 @default.
• W2032533435 hasAuthorship W2032533435A5029314765 @default.
• W2032533435 hasAuthorship W2032533435A5049448925 @default.
• W2032533435 hasAuthorship W2032533435A5050613763 @default.
• W2032533435 hasAuthorship W2032533435A5051198429 @default.
• W2032533435 hasAuthorship W2032533435A5060056480 @default.
• W2032533435 hasAuthorship W2032533435A5084922970 @default.
• W2032533435 hasAuthorship W2032533435A5089359104 @default.
• W2032533435 hasBestOaLocation W20325334351 @default.
• W2032533435 hasConcept C116569031 @default.
• W2032533435 hasConcept C170493617 @default.
• W2032533435 hasConcept C178790620 @default.
• W2032533435 hasConcept C179104552 @default.
• W2032533435 hasConcept C181199279 @default.
• W2032533435 hasConcept C185592680 @default.
• W2032533435 hasConcept C20705724 @default.
• W2032533435 hasConcept C2776217839 @default.
• W2032533435 hasConcept C2776294642 @default.
• W2032533435 hasConcept C2778063876 @default.
• W2032533435 hasConcept C2778455166 @default.
• W2032533435 hasConcept C2780534640 @default.
• W2032533435 hasConcept C2780768313 @default.
• W2032533435 hasConcept C2780874372 @default.
• W2032533435 hasConcept C2780997848 @default.
• W2032533435 hasConcept C55493867 @default.
• W2032533435 hasConcept C71240020 @default.
• W2032533435 hasConceptScore W2032533435C116569031 @default.
• W2032533435 hasConceptScore W2032533435C170493617 @default.
• W2032533435 hasConceptScore W2032533435C178790620 @default.
• W2032533435 hasConceptScore W2032533435C179104552 @default.
• W2032533435 hasConceptScore W2032533435C181199279 @default.
• W2032533435 hasConceptScore W2032533435C185592680 @default.
• W2032533435 hasConceptScore W2032533435C20705724 @default.
• W2032533435 hasConceptScore W2032533435C2776217839 @default.
• W2032533435 hasConceptScore W2032533435C2776294642 @default.
• W2032533435 hasConceptScore W2032533435C2778063876 @default.
• W2032533435 hasConceptScore W2032533435C2778455166 @default.
• W2032533435 hasConceptScore W2032533435C2780534640 @default.
• W2032533435 hasConceptScore W2032533435C2780768313 @default.
• W2032533435 hasConceptScore W2032533435C2780874372 @default.
• W2032533435 hasConceptScore W2032533435C2780997848 @default.
• W2032533435 hasConceptScore W2032533435C55493867 @default.
• W2032533435 hasConceptScore W2032533435C71240020 @default.

• next