FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032539152> ?p ?o ?g. }

• W2032539152 endingPage "2274" @default.
• W2032539152 startingPage "2265" @default.
• W2032539152 abstract "The detection of induced proteins after introduction of specific substrates in culture is of high interest for a comparative description of organisms growing under different conditions. In this study, protein-based stable isotope probing (Protein-SIP) is used for a fast and reliable detection of newly synthesized proteins in a substrate shift experiment. Therefore, Pseudomonas putida ML2 cells precultured on 12C-acetate and 12C-benzene, respectively, were incubated with 13C-benzene as a stable-isotope-labeled substrate. Protein samples from early to stationary growth phase were separated by one-dimensional gel electrophoresis (1-DE), subsequently tryptically digested, and analyzed by UPLC Orbitrap MS/MS measurements. Identified peptides from proteins involved in aerobic benzene degradation as well as from housekeeping proteins were chosen to calculate the labeling ratio (proportion of labeled protein to total protein) at different time points. A comparison of parameters from a nonlinear regression analysis of the calculated data enabled a clear differentiation between induced and constitutively expressed proteins. Thus, Protein-SIP has proven to be a valuable tool for quantitative analysis of induced proteins in substrate shift experiments." @default.
• W2032539152 created "2016-06-24" @default.
• W2032539152 creator A5009760942 @default.
• W2032539152 creator A5018412626 @default.
• W2032539152 creator A5035474246 @default.
• W2032539152 creator A5045874625 @default.
• W2032539152 creator A5068106192 @default.
• W2032539152 creator A5076028970 @default.
• W2032539152 creator A5081785538 @default.
• W2032539152 date "2011-05-20" @default.
• W2032539152 modified "2023-09-27" @default.
• W2032539152 title "Time resolved protein-based stable isotope probing (Protein-SIP) analysis allows quantification of induced proteins in substrate shift experiments" @default.
• W2032539152 cites W1491417004 @default.
• W2032539152 cites W1591150920 @default.
• W2032539152 cites W1750765063 @default.
• W2032539152 cites W1858398460 @default.
• W2032539152 cites W1900899030 @default.
• W2032539152 cites W1981593008 @default.
• W2032539152 cites W1985246182 @default.
• W2032539152 cites W2017369684 @default.
• W2032539152 cites W2022397401 @default.
• W2032539152 cites W2025422229 @default.
• W2032539152 cites W2026974599 @default.
• W2032539152 cites W2027848467 @default.
• W2032539152 cites W2039339985 @default.
• W2032539152 cites W2039471071 @default.
• W2032539152 cites W2040422637 @default.
• W2032539152 cites W2062737907 @default.
• W2032539152 cites W2064813444 @default.
• W2032539152 cites W2066321901 @default.
• W2032539152 cites W2066361645 @default.
• W2032539152 cites W2082751518 @default.
• W2032539152 cites W2084082495 @default.
• W2032539152 cites W2091619455 @default.
• W2032539152 cites W2097321270 @default.
• W2032539152 cites W2098707777 @default.
• W2032539152 cites W2106418531 @default.
• W2032539152 cites W2125553150 @default.
• W2032539152 cites W2140946052 @default.
• W2032539152 cites W2142880652 @default.
• W2032539152 cites W2160932397 @default.
• W2032539152 cites W2164917194 @default.
• W2032539152 doi "https://doi.org/10.1002/pmic.201000788" @default.
• W2032539152 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21598395" @default.
• W2032539152 hasPublicationYear "2011" @default.
• W2032539152 type Work @default.
• W2032539152 sameAs 2032539152 @default.
• W2032539152 citedByCount "42" @default.
• W2032539152 countsByYear W20325391522012 @default.
• W2032539152 countsByYear W20325391522013 @default.
• W2032539152 countsByYear W20325391522014 @default.
• W2032539152 countsByYear W20325391522015 @default.
• W2032539152 countsByYear W20325391522016 @default.
• W2032539152 countsByYear W20325391522017 @default.
• W2032539152 countsByYear W20325391522019 @default.
• W2032539152 countsByYear W20325391522020 @default.
• W2032539152 countsByYear W20325391522022 @default.
• W2032539152 countsByYear W20325391522023 @default.
• W2032539152 crossrefType "journal-article" @default.
• W2032539152 hasAuthorship W2032539152A5009760942 @default.
• W2032539152 hasAuthorship W2032539152A5018412626 @default.
• W2032539152 hasAuthorship W2032539152A5035474246 @default.
• W2032539152 hasAuthorship W2032539152A5045874625 @default.
• W2032539152 hasAuthorship W2032539152A5068106192 @default.
• W2032539152 hasAuthorship W2032539152A5076028970 @default.
• W2032539152 hasAuthorship W2032539152A5081785538 @default.
• W2032539152 hasConcept C104317684 @default.
• W2032539152 hasConcept C116084860 @default.
• W2032539152 hasConcept C162356407 @default.
• W2032539152 hasConcept C175605896 @default.
• W2032539152 hasConcept C181199279 @default.
• W2032539152 hasConcept C185592680 @default.
• W2032539152 hasConcept C18903297 @default.
• W2032539152 hasConcept C2777289219 @default.
• W2032539152 hasConcept C2777624702 @default.
• W2032539152 hasConcept C2780543182 @default.
• W2032539152 hasConcept C2780841973 @default.
• W2032539152 hasConcept C43617362 @default.
• W2032539152 hasConcept C46111723 @default.
• W2032539152 hasConcept C523546767 @default.
• W2032539152 hasConcept C54355233 @default.
• W2032539152 hasConcept C55493867 @default.
• W2032539152 hasConcept C80311884 @default.
• W2032539152 hasConcept C86803240 @default.
• W2032539152 hasConceptScore W2032539152C104317684 @default.
• W2032539152 hasConceptScore W2032539152C116084860 @default.
• W2032539152 hasConceptScore W2032539152C162356407 @default.
• W2032539152 hasConceptScore W2032539152C175605896 @default.
• W2032539152 hasConceptScore W2032539152C181199279 @default.
• W2032539152 hasConceptScore W2032539152C185592680 @default.
• W2032539152 hasConceptScore W2032539152C18903297 @default.
• W2032539152 hasConceptScore W2032539152C2777289219 @default.
• W2032539152 hasConceptScore W2032539152C2777624702 @default.
• W2032539152 hasConceptScore W2032539152C2780543182 @default.
• W2032539152 hasConceptScore W2032539152C2780841973 @default.
• W2032539152 hasConceptScore W2032539152C43617362 @default.
• W2032539152 hasConceptScore W2032539152C46111723 @default.
• W2032539152 hasConceptScore W2032539152C523546767 @default.

• next