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• W2032600564 abstract "A flow injection with pulsed amperometric detection for determination of doxycycline or chlortetracycline in pharmaceutical formulations is described. Doxycycline or chlortetracycline were studied at a gold rotating disk electrode with cyclic voltammetry as a function of pH of supporting electrolyte solution. The optimized PAD waveform parameters were obtained with a flow injection system. The optimized pulsed conditions of doxycycline were 1150 mV (versus Ag/AgCl reference electrode) detection potential (Edet) for 220 ms (150 ms delay time and 70 ms integration time), 1500 mV (versus Ag/AgCl reference electrode) oxidation potential (Eoxd) for 70 ms oxidation time (toxd) and 250 mV (versus Ag/AgCl reference electrode) reduction potentail (Ered) for 400 ms reactivation time (tred). The optimized pulsed conditions of chlortetracycline were 1050 mV (versus Ag/AgCl reference electrode) detection potential (Edet) for 300 ms (200 ms delay time and 100 ms integration time), 1300 mV (versus Ag/AgCl reference electrode) oxidation potential (Eoxd) for 70 ms oxidation time (toxd) and 250 mV (versus Ag/AgCl reference electrode) reduction potentail (Ered) for 400 ms reactivation time (tred). The optimized PAD waveform was applied to the determination of doxycycline hydrochloride and chlortetracycline hydrochloride standard solution and in pharmaceutical formulations. The linear dynamic ranges of doxycycline hydrochloride and chlortetracycline hydrochloride were 1 μM–0.1 mM. The sensitivity of this method was found to be 23 μA/mM for doxycycline hydrochloride and 33.76 μA/mM for chlortetracycline hydrochloride. The detection limit for both compounds is 1 μM. The doxycycline hydrochloride and chlortetracycline hydrochloride content in commercially available tablet dosage forms by the proposed method was comparable to those specified by the manufacturer." @default.
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• W2032600564 date "2004-12-01" @default.
• W2032600564 modified "2023-09-25" @default.
• W2032600564 title "Flow injection analysis of doxycycline or chlortetracycline in pharmaceutical formulations with pulsed amperometric detection" @default.
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• W2032600564 doi "https://doi.org/10.1016/j.talanta.2004.04.036" @default.
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