Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032821673> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2032821673 abstract "Background: The oncolytic potency of replication competent REOvirus has been demonstrated in various cancers. The activity is most pronounced in Kras mutant cancer cells, which account for 30-40 % of all cancers. Herein we simultaneously utilized isogenic human derived colorectal cancer cell lines that differ only by the presence of mutant Kras and normal rat intestinal epithelial cells with inducible Kras to evaluate whether the presence of oncogenic Kras alters the sensitivity of colon cancer cells to reovirus. Methods: Reovirus was obtained from Oncolytics Biotech Inc. Rat IEC9s harboring IPTG inducible mutant Ras oncogene (IEC-iKras) were treated with reovirus ± IPTG. The infectivity was maintained at a multiplicity of infection (MOI) of 2. MTT assay was performed at 72 hours to determine cell vability (CV). Similar experiments were also performed with HCT116 (Kras mut) and its isogenic derivative Hke3 [(Kras wild type (WT)]. Cells were cultured and treated in both serum rich (SR) and serum free (SF) media to determine the effect of growth factors on sensitivity to reovirus. Alterations in gene expression were determined by real time PCR and protein expression by western blot. Results: The activity of reovirus was observed in all cell lines studied. Reduction in CV was greater in Kras mutant HCT116 compared to WT Hke3 cells. Consistently, induction of Kras in IEC cells increased the potency of reovirus. Furthermore, this effect was more prominent in cells cultured in SF suggesting that growth factors in the serum may attenuate the effect of mutant Kras on reovirus sensitivity. Expression of the cell cycle regulator, p21 was preferentially increased upon reovirus infection in Kras mutant compared to WT cells, and in cells cultured in SF compared to SR conditions. Conclusion: Oncogenic Kras mutations increase the sensitivity of colon cancer cells to Reovirus. Clinical trials are underway testing reovirus in patients with Kras mutant metastatic colorectal cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4420. doi:1538-7445.AM2012-4420" @default.
- W2032821673 created "2016-06-24" @default.
- W2032821673 creator A5006031207 @default.
- W2032821673 creator A5029455802 @default.
- W2032821673 creator A5042470605 @default.
- W2032821673 creator A5046669683 @default.
- W2032821673 creator A5050975158 @default.
- W2032821673 creator A5069675476 @default.
- W2032821673 creator A5070227595 @default.
- W2032821673 date "2012-04-15" @default.
- W2032821673 modified "2023-09-26" @default.
- W2032821673 title "Abstract 4420: Serum regulates reovirus-mediated cytopathy in K-Ras activated colorectal cancer and intestinal epithelial cell lines" @default.
- W2032821673 doi "https://doi.org/10.1158/1538-7445.am2012-4420" @default.
- W2032821673 hasPublicationYear "2012" @default.
- W2032821673 type Work @default.
- W2032821673 sameAs 2032821673 @default.
- W2032821673 citedByCount "0" @default.
- W2032821673 crossrefType "proceedings-article" @default.
- W2032821673 hasAuthorship W2032821673A5006031207 @default.
- W2032821673 hasAuthorship W2032821673A5029455802 @default.
- W2032821673 hasAuthorship W2032821673A5042470605 @default.
- W2032821673 hasAuthorship W2032821673A5046669683 @default.
- W2032821673 hasAuthorship W2032821673A5050975158 @default.
- W2032821673 hasAuthorship W2032821673A5069675476 @default.
- W2032821673 hasAuthorship W2032821673A5070227595 @default.
- W2032821673 hasConcept C104317684 @default.
- W2032821673 hasConcept C121608353 @default.
- W2032821673 hasConcept C1491633281 @default.
- W2032821673 hasConcept C153911025 @default.
- W2032821673 hasConcept C203233990 @default.
- W2032821673 hasConcept C2776415932 @default.
- W2032821673 hasConcept C2781018059 @default.
- W2032821673 hasConcept C2781187634 @default.
- W2032821673 hasConcept C29537977 @default.
- W2032821673 hasConcept C3020616263 @default.
- W2032821673 hasConcept C502942594 @default.
- W2032821673 hasConcept C526805850 @default.
- W2032821673 hasConcept C54355233 @default.
- W2032821673 hasConcept C55493867 @default.
- W2032821673 hasConcept C62112901 @default.
- W2032821673 hasConcept C81885089 @default.
- W2032821673 hasConcept C82210918 @default.
- W2032821673 hasConcept C86803240 @default.
- W2032821673 hasConceptScore W2032821673C104317684 @default.
- W2032821673 hasConceptScore W2032821673C121608353 @default.
- W2032821673 hasConceptScore W2032821673C1491633281 @default.
- W2032821673 hasConceptScore W2032821673C153911025 @default.
- W2032821673 hasConceptScore W2032821673C203233990 @default.
- W2032821673 hasConceptScore W2032821673C2776415932 @default.
- W2032821673 hasConceptScore W2032821673C2781018059 @default.
- W2032821673 hasConceptScore W2032821673C2781187634 @default.
- W2032821673 hasConceptScore W2032821673C29537977 @default.
- W2032821673 hasConceptScore W2032821673C3020616263 @default.
- W2032821673 hasConceptScore W2032821673C502942594 @default.
- W2032821673 hasConceptScore W2032821673C526805850 @default.
- W2032821673 hasConceptScore W2032821673C54355233 @default.
- W2032821673 hasConceptScore W2032821673C55493867 @default.
- W2032821673 hasConceptScore W2032821673C62112901 @default.
- W2032821673 hasConceptScore W2032821673C81885089 @default.
- W2032821673 hasConceptScore W2032821673C82210918 @default.
- W2032821673 hasConceptScore W2032821673C86803240 @default.
- W2032821673 hasLocation W20328216731 @default.
- W2032821673 hasOpenAccess W2032821673 @default.
- W2032821673 hasPrimaryLocation W20328216731 @default.
- W2032821673 hasRelatedWork W1966963428 @default.
- W2032821673 hasRelatedWork W1980615608 @default.
- W2032821673 hasRelatedWork W2018857991 @default.
- W2032821673 hasRelatedWork W2037686237 @default.
- W2032821673 hasRelatedWork W2086925491 @default.
- W2032821673 hasRelatedWork W2266366869 @default.
- W2032821673 hasRelatedWork W2353278063 @default.
- W2032821673 hasRelatedWork W2364826228 @default.
- W2032821673 hasRelatedWork W2368783459 @default.
- W2032821673 hasRelatedWork W2377110681 @default.
- W2032821673 hasRelatedWork W2793510397 @default.
- W2032821673 hasRelatedWork W2885291746 @default.
- W2032821673 hasRelatedWork W2897103727 @default.
- W2032821673 hasRelatedWork W2909512161 @default.
- W2032821673 hasRelatedWork W2977601882 @default.
- W2032821673 hasRelatedWork W2980189876 @default.
- W2032821673 hasRelatedWork W3017755479 @default.
- W2032821673 hasRelatedWork W3081631363 @default.
- W2032821673 hasRelatedWork W3105474733 @default.
- W2032821673 hasRelatedWork W3141698530 @default.
- W2032821673 isParatext "false" @default.
- W2032821673 isRetracted "false" @default.
- W2032821673 magId "2032821673" @default.
- W2032821673 workType "article" @default.