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- W2032918807 abstract "Nuclear damage observed in X-irradiated thymocytes appears to be an expression of chromatin disaggregation. The development of the typical pycnotic morphology, which has been interpreted as chromatin condensation, is observed when irradiated cells are fixed for histological examination in the presence of a heavy metal. In the absence of heavy metal from the fixation procedure, however, the development of radiation damage in thymocyte nuclei is seen as a loss of the fine chromatin reticulum and replacement by structureless, homogeneous nuclei. These homogeneous nuclei were produced in unirradiated cells by incubation in hypotonic media as well as by suspending irradiated cells in an isotonic medium. Their formation in irradiated cells, however, was prevented or opposed by incubation in media with elevated sodium chloride concentrations (a treatment known to cause chromatin condensation). These hypertonic environments also reversed nuclei with established homogeneity back to a normal morphology. This evidence supports the idea that radiation damage in thymocyte nuclei involves chromatin disaggregation. It is suggested that irradiation causes altered membrane permeability which results in the loss of chromatin condensing agents (inorganic and organic cations) from the nucleus." @default.
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- W2032918807 date "1963-06-01" @default.
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- W2032918807 title "Reduction of nuclear damage in X-irradiated rat thymocytes by elevated salt concentrations" @default.
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- W2032918807 doi "https://doi.org/10.1016/0014-4827(63)90163-0" @default.
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