FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2032940004> ?p ?o ?g. }

• W2032940004 endingPage "311" @default.
• W2032940004 startingPage "300" @default.
• W2032940004 abstract "The synthesis, transport, and insertion of jimpy proteolipid protein and DM20 were studied in normal (158N) and jimpy (158JP) immortalized oligodendrocyte lines. Four different expression vectors encoding fusion proteins composed of native PLP and DM20 or jimpy PLP or DM20 were linked to enhanced green fluorescent protein (EGFP). All four transfected fusion proteins had similar distributions in the cell bodies and processes of the two cell types. Both normal and jimpy PLP-EGFP and DM20-EGFP were detected in both cell lines as far as 200 microM from the cell body, indicating synthesis and transport of mutated PLP and DM20 toward the plasma membrane. Immunocytochemistry of fixed normal and jimpy cells with the O10 antibody, which recognizes a conformationally sensitive PLP/DM20 epitope, confirmed that normal and jimpy PLP and DM20 were transported to the plasma membrane. Live staining of normal and jimpy cells transiently transfected with the native PLP showed positive staining, indicating PLP was correctly inserted into the membrane of both normal and jimpy oligodendrocytes. However, live staining of normal and jimpy cells transiently transfected with jimpy PLP showed no positive staining, indicating the mutated protein is abnormally inserted into the plasma membrane. Electrophysiological recordings of the resting membrane potential measured in the whole cell mode of the patch-clamp technique showed the absence of a developmentally regulated negative shift in the membrane potential in jimpy cells compared to normal native or immortalized oligodendrocytes. Treatment of 158N cells and native oligodendrocytes with dibutyryl cAMP (dbcAMP) caused morphological and biochemical differentiation, but failed to do so in 158JP cells, suggesting an abnormal signaling pathway in jimpy. The defect in cAMP signaling in jimpy oligodendrocytes was associated with the suppression of increase in mRNA level of the inducible cAMP early repressor (ICER). When the jimpy oligodendrocyte line was transfected with normal PLP or DM20 and exposed to dbcAMP, the cells failed to differentiate. This finding suggests that improper insertion of jimpy protein into the plasma membrane alters the membrane in such a way that certain signaling pathways are permanently altered. The abnormal insertion of jimpy PLP/DM20 into the plasma membrane may be the basis for the lack of cell signaling and abnormal resting potential in jimpy oligodendrocytes." @default.
• W2032940004 created "2016-06-24" @default.
• W2032940004 creator A5003711165 @default.
• W2032940004 creator A5023780227 @default.
• W2032940004 creator A5026824154 @default.
• W2032940004 creator A5055210348 @default.
• W2032940004 creator A5064700798 @default.
• W2032940004 creator A5071609899 @default.
• W2032940004 creator A5086200508 @default.
• W2032940004 creator A5090592750 @default.
• W2032940004 date "2002-10-23" @default.
• W2032940004 modified "2023-09-27" @default.
• W2032940004 title "Trafficking of PLP/DM20 and cAMP signaling in immortalized jimpy oligodendrocytes" @default.
• W2032940004 cites W143806508 @default.
• W2032940004 cites W1487464667 @default.
• W2032940004 cites W1506945781 @default.
• W2032940004 cites W1606017151 @default.
• W2032940004 cites W1822921207 @default.
• W2032940004 cites W1967440658 @default.
• W2032940004 cites W1971768862 @default.
• W2032940004 cites W1972060833 @default.
• W2032940004 cites W1972262740 @default.
• W2032940004 cites W1983856413 @default.
• W2032940004 cites W1984325422 @default.
• W2032940004 cites W1988079264 @default.
• W2032940004 cites W1988538057 @default.
• W2032940004 cites W1991686573 @default.
• W2032940004 cites W1996889271 @default.
• W2032940004 cites W2001049751 @default.
• W2032940004 cites W2006254842 @default.
• W2032940004 cites W2009667219 @default.
• W2032940004 cites W2015986188 @default.
• W2032940004 cites W2022167806 @default.
• W2032940004 cites W2025248673 @default.
• W2032940004 cites W2027701619 @default.
• W2032940004 cites W2028357105 @default.
• W2032940004 cites W2032598875 @default.
• W2032940004 cites W2033444235 @default.
• W2032940004 cites W2034446313 @default.
• W2032940004 cites W2038716730 @default.
• W2032940004 cites W2038726437 @default.
• W2032940004 cites W2047833547 @default.
• W2032940004 cites W2063016390 @default.
• W2032940004 cites W2067308634 @default.
• W2032940004 cites W2068502629 @default.
• W2032940004 cites W2068899813 @default.
• W2032940004 cites W2070193133 @default.
• W2032940004 cites W2076404268 @default.
• W2032940004 cites W2076957088 @default.
• W2032940004 cites W2079092972 @default.
• W2032940004 cites W2091834416 @default.
• W2032940004 cites W2098744846 @default.
• W2032940004 cites W2105518638 @default.
• W2032940004 cites W2126707631 @default.
• W2032940004 cites W2126907710 @default.
• W2032940004 cites W2141669165 @default.
• W2032940004 cites W2172027287 @default.
• W2032940004 cites W2399361076 @default.
• W2032940004 cites W242692584 @default.
• W2032940004 cites W9509700 @default.
• W2032940004 doi "https://doi.org/10.1002/glia.10122" @default.
• W2032940004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12420310" @default.
• W2032940004 hasPublicationYear "2002" @default.
• W2032940004 type Work @default.
• W2032940004 sameAs 2032940004 @default.
• W2032940004 citedByCount "19" @default.
• W2032940004 countsByYear W20329400042013 @default.
• W2032940004 countsByYear W20329400042014 @default.
• W2032940004 countsByYear W20329400042015 @default.
• W2032940004 countsByYear W20329400042017 @default.
• W2032940004 countsByYear W20329400042018 @default.
• W2032940004 countsByYear W20329400042022 @default.
• W2032940004 crossrefType "journal-article" @default.
• W2032940004 hasAuthorship W2032940004A5003711165 @default.
• W2032940004 hasAuthorship W2032940004A5023780227 @default.
• W2032940004 hasAuthorship W2032940004A5026824154 @default.
• W2032940004 hasAuthorship W2032940004A5055210348 @default.
• W2032940004 hasAuthorship W2032940004A5064700798 @default.
• W2032940004 hasAuthorship W2032940004A5071609899 @default.
• W2032940004 hasAuthorship W2032940004A5086200508 @default.
• W2032940004 hasAuthorship W2032940004A5090592750 @default.
• W2032940004 hasConcept C134018914 @default.
• W2032940004 hasConcept C153911025 @default.
• W2032940004 hasConcept C2776985911 @default.
• W2032940004 hasConcept C2777426553 @default.
• W2032940004 hasConcept C2778609137 @default.
• W2032940004 hasConcept C2780876595 @default.
• W2032940004 hasConcept C35866371 @default.
• W2032940004 hasConcept C529278444 @default.
• W2032940004 hasConcept C54009773 @default.
• W2032940004 hasConcept C54355233 @default.
• W2032940004 hasConcept C74864618 @default.
• W2032940004 hasConcept C7821365 @default.
• W2032940004 hasConcept C81885089 @default.
• W2032940004 hasConcept C86803240 @default.
• W2032940004 hasConcept C95444343 @default.
• W2032940004 hasConceptScore W2032940004C134018914 @default.
• W2032940004 hasConceptScore W2032940004C153911025 @default.

• next