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• W2033003670 abstract "The Pseudomonas aeruginosa FpvA receptor is a TonB-dependent outer membrane transport protein that catalyzes uptake of ferric pyoverdin across the outer membrane. Surprisingly, FpvA expressed in P. aeruginosa grown in an iron-deficient medium copurifies with a ligand X that we have characterized by UV, fluorescence, and mass spectrometry as being iron-free pyoverdin (apo-PaA). PaA was absent from FpvA purified from a PaA−deficient P. aeruginosa strain. The properties of ligand binding in vitro revealed very similar affinities of apo-PaA and ferric−PaA to FpvA. Fluorescence resonance energy transfer was used to study in vitro the formation of the FpvA−PaA−Fe complex in the presence of PaA−Fe or citrate−Fe. The circular dichroism spectrum of FpvA indicated a 57% β-structure content typical of porins and in agreement with the 3D structures of the siderophore receptors FhuA and FepA. In the absence of the protease's inhibitors, a truncated form of FpvA lacking 87 amino acids at its N-terminus was purified. This truncated form still bound PaA, and its β-sheet content was conserved. This N-terminal region displays significant homology to the N-terminal periplasmic extensions of FecA from Escherichia coli and PupB from Pseudomonas putida, which were previously shown to be involved in signal transduction. This suggests a similar function for FpvA. The mechanism of iron transport in P. aeruginosa via the pyoverdin pathway is discussed in the light of all these new findings." @default.
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• W2033003670 date "1999-06-30" @default.
• W2033003670 modified "2023-10-15" @default.
• W2033003670 title "Copurification of the FpvA Ferric Pyoverdin Receptor of<i>Pseudomonas</i><i>aeruginosa</i>with Its Iron-Free Ligand: Implications for Siderophore-Mediated Iron Transport" @default.
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• W2033003670 doi "https://doi.org/10.1021/bi990421x" @default.
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