FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033077457> ?p ?o ?g. }

• W2033077457 endingPage "e4417" @default.
• W2033077457 startingPage "e4417" @default.
• W2033077457 abstract "Putative living entities called nanobacteria (NB) are unusual for their small sizes (50–500 nm), pleomorphic nature, and accumulation of hydroxyapatite (HAP), and have been implicated in numerous diseases involving extraskeletal calcification. By adding precipitating ions to cell culture medium containing serum, mineral nanoparticles are generated that are morphologically and chemically identical to the so-called NB. These nanoparticles are shown here to be formed of amorphous mineral complexes containing calcium as well as other ions like carbonate, which then rapidly acquire phosphate, forming HAP. The main constituent proteins of serum-derived NB are albumin, fetuin-A, and apolipoprotein A1, but their involvement appears circumstantial since so-called NB from different body fluids harbor other proteins. Accordingly, by passage through various culture media, the protein composition of these particles can be modulated. Immunoblotting experiments reveal that antibodies deemed specific for NB react in fact with either albumin, fetuin-A, or both, indicating that previous studies using these reagents may have detected these serum proteins from the same as well as different species, with human tissue nanoparticles presumably absorbing bovine serum antigens from the culture medium. Both fetal bovine serum and human serum, used earlier by other investigators as sources of NB, paradoxically inhibit the formation of these entities, and this inhibition is trypsin-sensitive, indicating a role for proteins in this inhibitory process. Fetuin-A, and to a lesser degree albumin, inhibit nanoparticle formation, an inhibition that is overcome with time, ending with formation of the so-called NB. Together, these data demonstrate that NB are most likely formed by calcium or apatite crystallization inhibitors that are somehow overwhelmed by excess calcium or calcium phosphate found in culture medium or in body fluids, thereby becoming seeds for calcification. The structures described earlier as NB may thus represent remnants and by-products of physiological mechanisms used for calcium homeostasis, a concept which explains the vast body of NB literature as well as explains the true origin of NB as lifeless protein-mineralo entities with questionable role in pathogenesis." @default.
• W2033077457 created "2016-06-24" @default.
• W2033077457 creator A5004130387 @default.
• W2033077457 creator A5017860143 @default.
• W2033077457 creator A5023961314 @default.
• W2033077457 creator A5039271498 @default.
• W2033077457 creator A5043162896 @default.
• W2033077457 creator A5045799598 @default.
• W2033077457 date "2009-02-09" @default.
• W2033077457 modified "2023-10-03" @default.
• W2033077457 title "Putative Nanobacteria Represent Physiological Remnants and Culture By-Products of Normal Calcium Homeostasis" @default.
• W2033077457 cites W1487100945 @default.
• W2033077457 cites W1523172910 @default.
• W2033077457 cites W1966821238 @default.
• W2033077457 cites W1968506902 @default.
• W2033077457 cites W1968713120 @default.
• W2033077457 cites W1973948354 @default.
• W2033077457 cites W1981363992 @default.
• W2033077457 cites W1986453315 @default.
• W2033077457 cites W1986598651 @default.
• W2033077457 cites W1988246107 @default.
• W2033077457 cites W1995499344 @default.
• W2033077457 cites W1999893533 @default.
• W2033077457 cites W2004637219 @default.
• W2033077457 cites W2006155185 @default.
• W2033077457 cites W2006769626 @default.
• W2033077457 cites W2020007020 @default.
• W2033077457 cites W2021677417 @default.
• W2033077457 cites W2022055718 @default.
• W2033077457 cites W2023548466 @default.
• W2033077457 cites W2027400999 @default.
• W2033077457 cites W2028295894 @default.
• W2033077457 cites W2029496340 @default.
• W2033077457 cites W2030057384 @default.
• W2033077457 cites W2036610485 @default.
• W2033077457 cites W2043919846 @default.
• W2033077457 cites W2044267811 @default.
• W2033077457 cites W2044809585 @default.
• W2033077457 cites W2044892704 @default.
• W2033077457 cites W2045041306 @default.
• W2033077457 cites W2045230907 @default.
• W2033077457 cites W2047223499 @default.
• W2033077457 cites W2048695991 @default.
• W2033077457 cites W2051613518 @default.
• W2033077457 cites W2057150100 @default.
• W2033077457 cites W2062274491 @default.
• W2033077457 cites W2064332288 @default.
• W2033077457 cites W2068343592 @default.
• W2033077457 cites W2069212716 @default.
• W2033077457 cites W2069605751 @default.
• W2033077457 cites W2076095058 @default.
• W2033077457 cites W2076249265 @default.
• W2033077457 cites W2078669646 @default.
• W2033077457 cites W2081455630 @default.
• W2033077457 cites W2083356066 @default.
• W2033077457 cites W2084054365 @default.
• W2033077457 cites W2087060791 @default.
• W2033077457 cites W2087424173 @default.
• W2033077457 cites W2091275004 @default.
• W2033077457 cites W2092325416 @default.
• W2033077457 cites W2094712140 @default.
• W2033077457 cites W2104103518 @default.
• W2033077457 cites W2107940734 @default.
• W2033077457 cites W2115020939 @default.
• W2033077457 cites W2117349263 @default.
• W2033077457 cites W2136141583 @default.
• W2033077457 cites W2140769845 @default.
• W2033077457 cites W2142630168 @default.
• W2033077457 cites W2142754305 @default.
• W2033077457 cites W2147825225 @default.
• W2033077457 cites W2148587057 @default.
• W2033077457 cites W2151583254 @default.
• W2033077457 cites W2154283763 @default.
• W2033077457 cites W2156828519 @default.
• W2033077457 cites W2165985852 @default.
• W2033077457 cites W2167455623 @default.
• W2033077457 cites W2167680520 @default.
• W2033077457 cites W2286644986 @default.
• W2033077457 doi "https://doi.org/10.1371/journal.pone.0004417" @default.
• W2033077457 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2636888" @default.
• W2033077457 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19198665" @default.
• W2033077457 hasPublicationYear "2009" @default.
• W2033077457 type Work @default.
• W2033077457 sameAs 2033077457 @default.
• W2033077457 citedByCount "88" @default.
• W2033077457 countsByYear W20330774572012 @default.
• W2033077457 countsByYear W20330774572013 @default.
• W2033077457 countsByYear W20330774572014 @default.
• W2033077457 countsByYear W20330774572015 @default.
• W2033077457 countsByYear W20330774572016 @default.
• W2033077457 countsByYear W20330774572017 @default.
• W2033077457 countsByYear W20330774572018 @default.
• W2033077457 countsByYear W20330774572019 @default.
• W2033077457 countsByYear W20330774572020 @default.
• W2033077457 countsByYear W20330774572022 @default.
• W2033077457 countsByYear W20330774572023 @default.
• W2033077457 crossrefType "journal-article" @default.
• W2033077457 hasAuthorship W2033077457A5004130387 @default.

• next