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- W2033136941 startingPage "1184" @default.
- W2033136941 abstract "Protoplasts obtained from corn (Zea mays) suspension cells were studied using the whole cell patch-clamp technique. One time-independent current, as well as two time-dependent currents were identified. All three currents were reduced by tetraethylammonium (9 millimolar), a K(+) channel blocker. The time-independent current had a nearly linear current-voltage relationship and its reversal potential, defined as the voltage at which there is zero current, was highly dependent on the extracellular potassium concentration. One of the two time-dependent currents was activated, with rapid kinetics, by membrane hyperpolarization to potentials more negative than -100 millivolts. The second time-dependent current was activated with a sigmoidal time course by membrane depolarization to potentials more positive than -60 millivolts. It exhibited no inactivation and was carried primarily by potassium ions. These characteristics suggest that this latter current is caused by the voltage-dependent opening of delayed-rectifier K(+) channels. These three currents, which are not generated by the plasmalemma H(+)-ATPase, are likely to assist in the regulation of the cellular K(+) fluxes and membrane potential." @default.
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- W2033136941 date "1989-04-01" @default.
- W2033136941 modified "2023-10-18" @default.
- W2033136941 title "Characterization of Potassium-Dependent Currents in Protoplasts of Corn Suspension Cells" @default.
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- W2033136941 doi "https://doi.org/10.1104/pp.89.4.1184" @default.
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