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- W2033142009 abstract "Abstract An extract of bovine endometrium was found to decrease 3 H-thymidine incorporation into DNA of HeLa cells, but also, although to a lesser extent, of Ehrlich and Novikoff cells. Thymidine in the extract was responsible for about 80% of this inhibition. No inhibitor strictly specific for HeLa cells could be detected. An extract of human endometrium decreased, while an extract of human myometrium increased 3 H-thymidine incorporation into DNA of HeLa cells. The inhibitory effect of the endometrial extract was also observed on Ehrlich and Novikoff cells. The endometrial extract contained thymidine which was absent from the myometrial extract; thymidine in the endometrial extract was, however, a preparation artefact. Both human endometrial and myometrial extracts possessed an enzymic activity that converted thymidine into thymine; at high doses, the myometrial extract also decreased 3 H-thymidine incorporation into DNA of HeLa cells. Addition of unlabelled thymidine to the incubation medium has not the same inhibitory action in different kinds of cells on 3 H-thymidine incorporation into DNA. HeLa cells are very sensitive to this dilution effect. The differences in behavior of various cellular systems can be wrongly interpreted as the result of the action of a tissue-specific inhibitor." @default.
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- W2033142009 date "1975-09-01" @default.
- W2033142009 modified "2023-10-16" @default.
- W2033142009 title "Identification of the inhibitor of labelled thymidine incorporation into HeLa cell DNA present in endometrial extract" @default.
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- W2033142009 doi "https://doi.org/10.1016/0014-2964(75)90101-2" @default.
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