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• W2033142014 abstract "In single-particle electron microscopy (EM), multiple micrographs of identical macromolecular structures or complexes are taken from various viewing angles to obtain a 3D reconstruction. A high-quality EM reconstruction typically requires several thousand to several million images. Therefore, an automated pipeline for performing computations on many images becomes indispensable. In this paper, we propose a modified cross-correlation method to align a large number of images from the same class in single-particle electron microscopy of highly nonspherical structures, and show how this method fits into a larger automated pipeline for the discovery of 3D structures. Our modification uses a probability density in full planar position and orientation, akin to the pose densities used in Simultaneous Localization and Mapping (SLAM) and Assembly Automation. Using this alignment and a subsequent averaging process, high signal-to-noise ratio (SNR) images representing each class of viewing angles are obtained for reconstruction algorithms. In the proposed method, first we coarsely align projection images, and then realign the resulting images using the cross correlation (CC) method. The coarse alignment is obtained by matching the centers of mass and the principal axes of the images. The distribution of misalignment in this coarse alignment is estimated using the statistical properties of the additive background noise. As a consequence, the search space for realignment in the CC method is reduced. Additionally, in order to overcome the false peak problems in the CC, we use artificially blurred images for the early stage of the iteration and segment the intermediate result from every iteration step. The proposed approach is demonstrated on synthetic noisy images of GroEL/ES." @default.
• W2033142014 created "2016-06-24" @default.
• W2033142014 creator A5012775344 @default.
• W2033142014 creator A5075962780 @default.
• W2033142014 date "2014-07-01" @default.
• W2033142014 modified "2023-09-23" @default.
• W2033142014 title "An Assembly Automation Approach to Alignment of Noncircular Projections in Electron Microscopy" @default.
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• W2033142014 doi "https://doi.org/10.1109/tase.2013.2295398" @default.
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