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• W2033170435 startingPage "1425" @default.
• W2033170435 abstract "RecF pathway proteins play an important role in the restart of stalled replication and DNA repair in prokaryotes. Following DNA damage, RecF, RecR, and RecO initiate homologous recombination (HR) by loading of the RecA recombinase on single-stranded (ss) DNA, protected by ssDNA-binding protein. The specific role of RecF in this process is not well understood. Previous studies have proposed that RecF directs the RecOR complex to boundaries of damaged DNA regions by recognizing single-stranded/double-stranded (ss/ds) DNA junctions. RecF belongs to ABC-type ATPases, which function through an ATP-dependent dimerization. Here, we demonstrate that the RecF of <i>Deinococcus radiodurans</i> interacts with DNA as an ATP-dependent dimer, and that the DNA binding and ATPase activity of RecF depend on both the structure of DNA substrate, and the presence of RecR. We found that RecR interacts as a tetramer with the RecF dimer. RecR increases the RecF affinity to dsDNA without stimulating ATP hydrolysis but destabilizes RecF binding to ssDNA and dimerization, likely due to increasing the ATPase rate. The DNA-dependent binding of RecR to the RecF-DNA complex occurs through specific protein-protein interactions without significant contributions from RecR-DNA interactions. Finally, RecF neither alone nor in complex with RecR preferentially binds to the ss/dsDNA junction. Our data suggest that the specificity of the RecFOR complex toward the boundaries of DNA damaged regions may result from a network of protein-protein and DNA-protein interactions, rather than a simple recognition of the ss/dsDNA junction by RecF." @default.
• W2033170435 created "2016-06-24" @default.
• W2033170435 creator A5054238506 @default.
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• W2033170435 date "2009-01-01" @default.
• W2033170435 modified "2023-10-17" @default.
• W2033170435 title "RecR-mediated Modulation of RecF Dimer Specificity for Single- and Double-stranded DNA" @default.
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• W2033170435 doi "https://doi.org/10.1074/jbc.m806378200" @default.
• W2033170435 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2615518" @default.
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