FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033193394> ?p ?o ?g. }

• W2033193394 endingPage "355" @default.
• W2033193394 startingPage "345" @default.
• W2033193394 abstract "As an acute-phase protein, human C-reactive protein (CRP) is clinically important. CRPs were purified from several samples in six different pathological conditions, where their levels ranged from 22 to 342 μg/ml. Small, but significant, variations in electrophoretic mobilities on native PAGE suggested differences in molecular mass, charge and/or shape. Following separation by SDS/PAGE, they showed single subunits with some differences in their molecular masses ranging between 27 and 30.5 kDa, but for a particular disease, the mobility was the same for CRPs purified from multiple individuals or pooled sera. Isoelectric focusing (IEF) also indicated that the purified CRPs differed from each other. Glycosylation was demonstrated in these purified CRPs by Digoxigenin kits, neuraminidase treatment and binding with lectins. The presence of N-linked sugar moiety was confirmed by N-glycosidase F digestion. The presence of sialic acid, glucose, galactose and mannose has been demonstrated by gas liquid chromatography, mass spectroscopic and fluorimetric analysis. Matrix-assisted laser-desorption ionization analysis of the tryptic digests of three CRPs showed systematic absence of two peptide fragments, one at the N-terminus and the other near the C-terminus. Model-building suggested that the loss of these fragments exposed two potential glycosylation sites on a cleft floor keeping the protein–protein interactions in pentraxins and calcium-dependent phosphorylcholine-binding qualitatively unaffected. Thus we have convincingly demonstrated that human CRP is glycosylated in some pathological conditions." @default.
• W2033193394 created "2016-06-24" @default.
• W2033193394 creator A5020904549 @default.
• W2033193394 creator A5037331641 @default.
• W2033193394 creator A5073565228 @default.
• W2033193394 creator A5077122165 @default.
• W2033193394 creator A5084594210 @default.
• W2033193394 date "2003-07-15" @default.
• W2033193394 modified "2023-10-10" @default.
• W2033193394 title "Induction of glycosylation in human C-reactive protein under different pathological conditions" @default.
• W2033193394 cites W107253658 @default.
• W2033193394 cites W1522933334 @default.
• W2033193394 cites W1553832540 @default.
• W2033193394 cites W1579377762 @default.
• W2033193394 cites W1591789442 @default.
• W2033193394 cites W1597973709 @default.
• W2033193394 cites W1852206174 @default.
• W2033193394 cites W1874706679 @default.
• W2033193394 cites W1969576580 @default.
• W2033193394 cites W1993799487 @default.
• W2033193394 cites W1998854631 @default.
• W2033193394 cites W2010826690 @default.
• W2033193394 cites W2024191111 @default.
• W2033193394 cites W2031018826 @default.
• W2033193394 cites W2034790535 @default.
• W2033193394 cites W2047522139 @default.
• W2033193394 cites W2048985212 @default.
• W2033193394 cites W2063572291 @default.
• W2033193394 cites W2065219322 @default.
• W2033193394 cites W2075568133 @default.
• W2033193394 cites W2083453910 @default.
• W2033193394 cites W2092233764 @default.
• W2033193394 cites W2101856144 @default.
• W2033193394 cites W2106322549 @default.
• W2033193394 cites W2111301002 @default.
• W2033193394 cites W2161465441 @default.
• W2033193394 cites W2167973356 @default.
• W2033193394 cites W2183362862 @default.
• W2033193394 cites W2188885359 @default.
• W2033193394 cites W2317184793 @default.
• W2033193394 cites W2417250549 @default.
• W2033193394 cites W329437070 @default.
• W2033193394 doi "https://doi.org/10.1042/bj20021701" @default.
• W2033193394 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223501" @default.
• W2033193394 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12693993" @default.
• W2033193394 hasPublicationYear "2003" @default.
• W2033193394 type Work @default.
• W2033193394 sameAs 2033193394 @default.
• W2033193394 citedByCount "68" @default.
• W2033193394 countsByYear W20331933942012 @default.
• W2033193394 countsByYear W20331933942013 @default.
• W2033193394 countsByYear W20331933942014 @default.
• W2033193394 countsByYear W20331933942015 @default.
• W2033193394 countsByYear W20331933942016 @default.
• W2033193394 countsByYear W20331933942017 @default.
• W2033193394 countsByYear W20331933942018 @default.
• W2033193394 countsByYear W20331933942019 @default.
• W2033193394 countsByYear W20331933942020 @default.
• W2033193394 countsByYear W20331933942021 @default.
• W2033193394 countsByYear W20331933942022 @default.
• W2033193394 countsByYear W20331933942023 @default.
• W2033193394 crossrefType "journal-article" @default.
• W2033193394 hasAuthorship W2033193394A5020904549 @default.
• W2033193394 hasAuthorship W2033193394A5037331641 @default.
• W2033193394 hasAuthorship W2033193394A5073565228 @default.
• W2033193394 hasAuthorship W2033193394A5077122165 @default.
• W2033193394 hasAuthorship W2033193394A5084594210 @default.
• W2033193394 hasBestOaLocation W20331933942 @default.
• W2033193394 hasConcept C108625454 @default.
• W2033193394 hasConcept C179247698 @default.
• W2033193394 hasConcept C181199279 @default.
• W2033193394 hasConcept C185592680 @default.
• W2033193394 hasConcept C2775887612 @default.
• W2033193394 hasConcept C2777313579 @default.
• W2033193394 hasConcept C2780115458 @default.
• W2033193394 hasConcept C50476208 @default.
• W2033193394 hasConcept C55493867 @default.
• W2033193394 hasConcept C58121356 @default.
• W2033193394 hasConcept C96294017 @default.
• W2033193394 hasConceptScore W2033193394C108625454 @default.
• W2033193394 hasConceptScore W2033193394C179247698 @default.
• W2033193394 hasConceptScore W2033193394C181199279 @default.
• W2033193394 hasConceptScore W2033193394C185592680 @default.
• W2033193394 hasConceptScore W2033193394C2775887612 @default.
• W2033193394 hasConceptScore W2033193394C2777313579 @default.
• W2033193394 hasConceptScore W2033193394C2780115458 @default.
• W2033193394 hasConceptScore W2033193394C50476208 @default.
• W2033193394 hasConceptScore W2033193394C55493867 @default.
• W2033193394 hasConceptScore W2033193394C58121356 @default.
• W2033193394 hasConceptScore W2033193394C96294017 @default.
• W2033193394 hasIssue "2" @default.
• W2033193394 hasLocation W20331933941 @default.
• W2033193394 hasLocation W20331933942 @default.
• W2033193394 hasLocation W20331933943 @default.
• W2033193394 hasLocation W20331933944 @default.
• W2033193394 hasOpenAccess W2033193394 @default.
• W2033193394 hasPrimaryLocation W20331933941 @default.
• W2033193394 hasRelatedWork W1508473025 @default.

• next