Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033206990> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2033206990 endingPage "151" @default.
- W2033206990 startingPage "145" @default.
- W2033206990 abstract "A phenotypic method was developed to test mutations in the human cytomegalovirus (HCMV) DNA polymerase gene (UL54) suspected to confer resistance to foscarnet. This method was used to determine the biochemical phenotype of wild-type and mutated HCMV DNA polymerases that had been synthesised in vitro as follows. The UL54 genes were amplified from foscarnet-resistant and -sensitive isolates by PCR and the products were cloned into an expression vector under the control of a T7 promoter. Mutations were introduced by site-directed mutagenesis into wild-type gene UL54 and then polymerases were synthesised by using a commercially available coupled transcription/translation system. Polymerase activity was measured with and without foscarnet by detecting the incorporation of digoxigenin-labelled nucleotides into the growing DNA chain. The results of this non-radioactive assay were consistent with those obtained with the conventional radioactive assay. It was found that the activity of polymerases containing the V715M or E756K mutations was inhibited by foscarnet at concentrations 70- and 30-fold higher than that of wild-type polymerase, respectively. Change N495K and a combination of changes K415R and S291P, both observed in foscarnet-resistant isolates, induced a 5- and 10-fold decrease in susceptibility to foscarnet, respectively. This non-radioactive phenotypic assay could be useful for the characterisation of mutations that confer HCMV resistance to foscarnet." @default.
- W2033206990 created "2016-06-24" @default.
- W2033206990 creator A5012558883 @default.
- W2033206990 creator A5016508902 @default.
- W2033206990 creator A5047940189 @default.
- W2033206990 creator A5048095232 @default.
- W2033206990 creator A5078717533 @default.
- W2033206990 creator A5082698273 @default.
- W2033206990 creator A5090199618 @default.
- W2033206990 date "2005-05-01" @default.
- W2033206990 modified "2023-10-09" @default.
- W2033206990 title "Phenotypic characterisation of cytomegalovirus DNA polymerase: a method to study cytomegalovirus isolates resistant to foscarnet" @default.
- W2033206990 cites W1580498901 @default.
- W2033206990 cites W1770249567 @default.
- W2033206990 cites W1893350708 @default.
- W2033206990 cites W1976466959 @default.
- W2033206990 cites W1994589854 @default.
- W2033206990 cites W2007308294 @default.
- W2033206990 cites W2026049852 @default.
- W2033206990 cites W2040801518 @default.
- W2033206990 cites W2055016867 @default.
- W2033206990 cites W2061388052 @default.
- W2033206990 cites W2072275945 @default.
- W2033206990 cites W2088972589 @default.
- W2033206990 cites W2105881499 @default.
- W2033206990 cites W2114786252 @default.
- W2033206990 cites W2119793434 @default.
- W2033206990 cites W2130684389 @default.
- W2033206990 cites W2135837458 @default.
- W2033206990 cites W2160993726 @default.
- W2033206990 cites W2167351117 @default.
- W2033206990 cites W2611675546 @default.
- W2033206990 cites W39118261 @default.
- W2033206990 doi "https://doi.org/10.1016/j.jviromet.2005.01.005" @default.
- W2033206990 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15794984" @default.
- W2033206990 hasPublicationYear "2005" @default.
- W2033206990 type Work @default.
- W2033206990 sameAs 2033206990 @default.
- W2033206990 citedByCount "12" @default.
- W2033206990 countsByYear W20332069902012 @default.
- W2033206990 countsByYear W20332069902013 @default.
- W2033206990 countsByYear W20332069902014 @default.
- W2033206990 crossrefType "journal-article" @default.
- W2033206990 hasAuthorship W2033206990A5012558883 @default.
- W2033206990 hasAuthorship W2033206990A5016508902 @default.
- W2033206990 hasAuthorship W2033206990A5047940189 @default.
- W2033206990 hasAuthorship W2033206990A5048095232 @default.
- W2033206990 hasAuthorship W2033206990A5078717533 @default.
- W2033206990 hasAuthorship W2033206990A5082698273 @default.
- W2033206990 hasAuthorship W2033206990A5090199618 @default.
- W2033206990 hasConcept C104317684 @default.
- W2033206990 hasConcept C153911025 @default.
- W2033206990 hasConcept C159047783 @default.
- W2033206990 hasConcept C2522874641 @default.
- W2033206990 hasConcept C2756471 @default.
- W2033206990 hasConcept C2776724271 @default.
- W2033206990 hasConcept C2777965375 @default.
- W2033206990 hasConcept C2779820661 @default.
- W2033206990 hasConcept C49105822 @default.
- W2033206990 hasConcept C54355233 @default.
- W2033206990 hasConcept C82381507 @default.
- W2033206990 hasConcept C86803240 @default.
- W2033206990 hasConceptScore W2033206990C104317684 @default.
- W2033206990 hasConceptScore W2033206990C153911025 @default.
- W2033206990 hasConceptScore W2033206990C159047783 @default.
- W2033206990 hasConceptScore W2033206990C2522874641 @default.
- W2033206990 hasConceptScore W2033206990C2756471 @default.
- W2033206990 hasConceptScore W2033206990C2776724271 @default.
- W2033206990 hasConceptScore W2033206990C2777965375 @default.
- W2033206990 hasConceptScore W2033206990C2779820661 @default.
- W2033206990 hasConceptScore W2033206990C49105822 @default.
- W2033206990 hasConceptScore W2033206990C54355233 @default.
- W2033206990 hasConceptScore W2033206990C82381507 @default.
- W2033206990 hasConceptScore W2033206990C86803240 @default.
- W2033206990 hasIssue "2" @default.
- W2033206990 hasLocation W20332069901 @default.
- W2033206990 hasLocation W20332069902 @default.
- W2033206990 hasLocation W20332069903 @default.
- W2033206990 hasLocation W20332069904 @default.
- W2033206990 hasOpenAccess W2033206990 @default.
- W2033206990 hasPrimaryLocation W20332069901 @default.
- W2033206990 hasRelatedWork W1542157290 @default.
- W2033206990 hasRelatedWork W1699324194 @default.
- W2033206990 hasRelatedWork W1971780220 @default.
- W2033206990 hasRelatedWork W2006791504 @default.
- W2033206990 hasRelatedWork W2035628333 @default.
- W2033206990 hasRelatedWork W2141390486 @default.
- W2033206990 hasRelatedWork W2403997462 @default.
- W2033206990 hasRelatedWork W2408434996 @default.
- W2033206990 hasRelatedWork W2411851185 @default.
- W2033206990 hasRelatedWork W4292589977 @default.
- W2033206990 hasVolume "125" @default.
- W2033206990 isParatext "false" @default.
- W2033206990 isRetracted "false" @default.
- W2033206990 magId "2033206990" @default.
- W2033206990 workType "article" @default.