Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033320450> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2033320450 endingPage "26" @default.
- W2033320450 startingPage "19" @default.
- W2033320450 abstract "We used DNA transfection and protein introduction techniques to investigate the pressure tolerance of cytoskeletal structures in pectoral fin cells derived from the deep-sea fish Simenchelys parasiticus (habitat depth, 366–2,630 m). The deep-sea fish cells have G418 resistance. The cell number increased until day 6 of cultivation and all cells had died by day 35 when cultured in 35-mm Petri dishes in medium containing G418. Enhanced yellow fluorescent protein-tagged human β-actin (EYFP-actin) was stably expressed by 1 in 100,000 deep-sea fish cells. Because almost none of the EYFP-actin was incorporated into actin filaments of the cells, we replaced the relatively large EYFP tag with a chemical fluorescent compound and succeeded in incorporating fluorescently labeled rabbit actins into the deep-sea fish actin filaments. Most of the filament structure in the cells with rabbit actin inserted underwent depolymerization when subjected to pressure of 100 MPa for 20 min, in contrast to control cells. There were no differences in the tubulin filament structure between control cells and deep-sea fish cells with fluorescein-labeled bovine tubulin inserted after the application of pressure ranging from 40 to 100 MPa for 20 min." @default.
- W2033320450 created "2016-06-24" @default.
- W2033320450 creator A5062929264 @default.
- W2033320450 creator A5069578384 @default.
- W2033320450 date "2007-10-16" @default.
- W2033320450 modified "2023-10-14" @default.
- W2033320450 title "Piezotolerance of the cytoskeletal structure in cultured deep-sea fish cells using DNA transfection and protein introduction techniques" @default.
- W2033320450 cites W1965560996 @default.
- W2033320450 cites W1987406129 @default.
- W2033320450 cites W2038126991 @default.
- W2033320450 cites W2039462617 @default.
- W2033320450 cites W2041066505 @default.
- W2033320450 cites W2043159221 @default.
- W2033320450 cites W2048233025 @default.
- W2033320450 cites W2049162036 @default.
- W2033320450 cites W2063134564 @default.
- W2033320450 cites W2067714035 @default.
- W2033320450 cites W2071969228 @default.
- W2033320450 cites W2077747135 @default.
- W2033320450 cites W2083953853 @default.
- W2033320450 cites W2083996644 @default.
- W2033320450 cites W2087010662 @default.
- W2033320450 cites W2093721037 @default.
- W2033320450 cites W2403010888 @default.
- W2033320450 cites W2468327850 @default.
- W2033320450 cites W4231964183 @default.
- W2033320450 cites W564422668 @default.
- W2033320450 doi "https://doi.org/10.1007/s10616-007-9099-7" @default.
- W2033320450 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2151967" @default.
- W2033320450 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19002837" @default.
- W2033320450 hasPublicationYear "2007" @default.
- W2033320450 type Work @default.
- W2033320450 sameAs 2033320450 @default.
- W2033320450 citedByCount "6" @default.
- W2033320450 countsByYear W20333204502015 @default.
- W2033320450 countsByYear W20333204502019 @default.
- W2033320450 countsByYear W20333204502020 @default.
- W2033320450 countsByYear W20333204502021 @default.
- W2033320450 crossrefType "journal-article" @default.
- W2033320450 hasAuthorship W2033320450A5062929264 @default.
- W2033320450 hasAuthorship W2033320450A5069578384 @default.
- W2033320450 hasBestOaLocation W20333204502 @default.
- W2033320450 hasConcept C125705527 @default.
- W2033320450 hasConcept C14228908 @default.
- W2033320450 hasConcept C142669718 @default.
- W2033320450 hasConcept C1491633281 @default.
- W2033320450 hasConcept C153911025 @default.
- W2033320450 hasConcept C54009773 @default.
- W2033320450 hasConcept C54355233 @default.
- W2033320450 hasConcept C55493867 @default.
- W2033320450 hasConcept C81885089 @default.
- W2033320450 hasConcept C86803240 @default.
- W2033320450 hasConcept C95444343 @default.
- W2033320450 hasConceptScore W2033320450C125705527 @default.
- W2033320450 hasConceptScore W2033320450C14228908 @default.
- W2033320450 hasConceptScore W2033320450C142669718 @default.
- W2033320450 hasConceptScore W2033320450C1491633281 @default.
- W2033320450 hasConceptScore W2033320450C153911025 @default.
- W2033320450 hasConceptScore W2033320450C54009773 @default.
- W2033320450 hasConceptScore W2033320450C54355233 @default.
- W2033320450 hasConceptScore W2033320450C55493867 @default.
- W2033320450 hasConceptScore W2033320450C81885089 @default.
- W2033320450 hasConceptScore W2033320450C86803240 @default.
- W2033320450 hasConceptScore W2033320450C95444343 @default.
- W2033320450 hasIssue "1" @default.
- W2033320450 hasLocation W20333204501 @default.
- W2033320450 hasLocation W20333204502 @default.
- W2033320450 hasLocation W20333204503 @default.
- W2033320450 hasLocation W20333204504 @default.
- W2033320450 hasOpenAccess W2033320450 @default.
- W2033320450 hasPrimaryLocation W20333204501 @default.
- W2033320450 hasRelatedWork W2008589801 @default.
- W2033320450 hasRelatedWork W2024493059 @default.
- W2033320450 hasRelatedWork W2039495782 @default.
- W2033320450 hasRelatedWork W2119956045 @default.
- W2033320450 hasRelatedWork W2123485510 @default.
- W2033320450 hasRelatedWork W2594707584 @default.
- W2033320450 hasRelatedWork W2971551249 @default.
- W2033320450 hasRelatedWork W3127051863 @default.
- W2033320450 hasRelatedWork W3179330981 @default.
- W2033320450 hasRelatedWork W4307905277 @default.
- W2033320450 hasVolume "56" @default.
- W2033320450 isParatext "false" @default.
- W2033320450 isRetracted "false" @default.
- W2033320450 magId "2033320450" @default.
- W2033320450 workType "article" @default.