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- W2033322676 abstract "The active center of human glutaredoxin (hGrx1) shares a common thioredoxin fold and specific affinity for substrate glutathione (GSH) with natural glutathione peroxidase (GPx). hGrx1 was redesigned to introduce the catalytic selenocysteine residue to imitate the function of antioxidant selenoenzyme GPx in vivo. The human hGrx1 scaffold is a good candidate for potential medical application compared with other animal-originated protein scaffolds. Two consecutive rare codons (AGG-AGG) in the open reading frame of hGrx1 mRNA encoding Arg26–Arg27 residues can reduce seleno-hGrx1 expression level significantly in the Cys auxotrophic Escherichia coli strain BL21cysE51. Therefore, we optimized the rare codons, which resulted in a remarkable increase of the expression level in the Cys auxotrophic cells, which may be sufficient for future medical production. The engineered artificial selenoenzyme displays high GPx catalytic activity, rivaling that of some natural GPx proteins. Kinetic analysis of the engineered seleno-hGrx1 showed a typical ping-pong kinetic mechanism; its catalytic properties are similar to those of some naturally occurring GPx proteins." @default.
- W2033322676 created "2016-06-24" @default.
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- W2033322676 date "2012-01-01" @default.
- W2033322676 modified "2023-09-26" @default.
- W2033322676 title "Engineering human seleno-glutaredoxin containing consecutive rare codons as an artificial glutathione peroxidase" @default.
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- W2033322676 doi "https://doi.org/10.1007/s11434-011-4711-9" @default.
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