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- W2033366958 abstract "Various mycotoxins with strong carcinogenesis and toxicity are fatal threats in food safety, and require highly sensitive and high-throughput detections greatly. Herein a fluorescent competitive immunoassay microarray based on a non-fouling polymer brush, poly[(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) is explored to sensitively detect multiple mycotoxins with aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEN) as template targets. Due to uniformly large protein loading and high resistance to nonspecific protein absorption of the POEGMA-co-GMA brush, the optimal microarray exhibits wide dynamic ranges of three orders of magnitudes and low detection limits of 4, 4 and 3 pg mL−1, respectively, which is much better than that obtained with an epoxy-functionalized antigen microarray, and is comparable or even better than the conventional ELISA method. This work offers a powerful high-throughput tool to fast screening of toxins in food quality and environmental monitoring." @default.
- W2033366958 created "2016-06-24" @default.
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- W2033366958 date "2013-12-01" @default.
- W2033366958 modified "2023-09-25" @default.
- W2033366958 title "Sensitive competitive immunoassay of multiple mycotoxins with non-fouling antigen microarray" @default.
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- W2033366958 doi "https://doi.org/10.1016/j.bios.2013.06.037" @default.
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