FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033407116> ?p ?o ?g. }

• W2033407116 endingPage "56" @default.
• W2033407116 startingPage "48" @default.
• W2033407116 abstract "Phosphatidylinositol 4,5-bisphosphate (PtdIns 4,5-P2) is the substrate for phosphoinositide-phospholipase C (PLC) and is required for the function of several cardiac cell plasma membrane (sarcolemma, SL) proteins. PtdIns 4,5-P2 is synthesized in the SL membrane by coordinated and successive actions of PtdIns 4-kinase and PtdIns 4-phosphate 5-kinase. These kinases and the generation of PtdIns 4,5-P2 may be a factor in the cardiac dysfunction during pathophysiological conditions of oxidative stress. Therefore, we examined the effects of different reactive oxygen species (ROS) on the kinases' activities and subsequent generation of PtdIns 4,5-P2. Exposure to the xanthine-xanthine oxidase-ROS generating system significantly reduced both SL kinase activities. Superoxide dismutase did not prevent this inhibition; however, catalase significantly prevented the xanthine-xanthine oxidase induced inhibition. Treatment of SL with hydrogen peroxide (H2O2) resulted in inhibition of both the kinases, which was prevented by catalase and dithiothreitol (DTT). Hypochlorous acid also inhibited both the kinases, which was prevented by DTT. Deferoxamine (an iron chelator) and mannitol (an *OH scavenger) did not modify the H2O2-induced depression of the kinases, eliminating any role of *OH. Furthermore, the IC50 of H2O2 on PtdIns 4-kinase and PtdIns 4-P 5-kinase was 27 and 81 microM, respectively. In addition, inclusion of reduced glutathione in the assay of the kinases in the absence of H2O2 did not affect the activities of the kinases; however, oxidized glutathione induced a significant depression. Also, a significant decline of the PtdIns 4-kinase and PtdIns 4-P 5-kinase activities due to changing of the redox ratio was observed. Thiol modifiers (N-ethylmaleimide, methyl methanethiosulfonate, or p-chloromercuriphenylsulfonic acid) were detected to depress the kinases' activities, which were substantially prevented by DTT. The results suggest that functionally critical thiol groups may be associated with PtdIns 4-kinase and PtdIns 4-P 5-kinase and that changes of their redox state by ROS can impair their activities, which may be an important factor in the oxidant-induced cardiac dysfunction." @default.
• W2033407116 created "2016-06-24" @default.
• W2033407116 creator A5011896624 @default.
• W2033407116 creator A5031000915 @default.
• W2033407116 creator A5051559153 @default.
• W2033407116 creator A5068857226 @default.
• W2033407116 creator A5085983408 @default.
• W2033407116 date "2000-10-01" @default.
• W2033407116 modified "2023-09-27" @default.
• W2033407116 title "Oxidants Depress the Synthesis of Phosphatidylinositol 4,5-Bisphosphate in Heart Sarcolemma" @default.
• W2033407116 cites W1556814137 @default.
• W2033407116 cites W1589615575 @default.
• W2033407116 cites W1859184462 @default.
• W2033407116 cites W1964622351 @default.
• W2033407116 cites W1967701706 @default.
• W2033407116 cites W1968557891 @default.
• W2033407116 cites W1968719134 @default.
• W2033407116 cites W1970315506 @default.
• W2033407116 cites W1973330766 @default.
• W2033407116 cites W1973475159 @default.
• W2033407116 cites W1977350980 @default.
• W2033407116 cites W1997480152 @default.
• W2033407116 cites W1998507923 @default.
• W2033407116 cites W2000294953 @default.
• W2033407116 cites W2014545304 @default.
• W2033407116 cites W2019018307 @default.
• W2033407116 cites W2019759867 @default.
• W2033407116 cites W2020126595 @default.
• W2033407116 cites W2020965089 @default.
• W2033407116 cites W2030585484 @default.
• W2033407116 cites W2036451354 @default.
• W2033407116 cites W2036708447 @default.
• W2033407116 cites W2038322373 @default.
• W2033407116 cites W2045951786 @default.
• W2033407116 cites W2050923205 @default.
• W2033407116 cites W2053467384 @default.
• W2033407116 cites W2064062531 @default.
• W2033407116 cites W2069563551 @default.
• W2033407116 cites W2071786252 @default.
• W2033407116 cites W2075476485 @default.
• W2033407116 cites W2080997681 @default.
• W2033407116 cites W2081713598 @default.
• W2033407116 cites W2083281709 @default.
• W2033407116 cites W208442758 @default.
• W2033407116 cites W2088449995 @default.
• W2033407116 cites W2091903932 @default.
• W2033407116 cites W2113144597 @default.
• W2033407116 cites W2139603464 @default.
• W2033407116 cites W2144033216 @default.
• W2033407116 cites W2162673362 @default.
• W2033407116 cites W2163749170 @default.
• W2033407116 cites W2167781998 @default.
• W2033407116 cites W2284280435 @default.
• W2033407116 cites W93783570 @default.
• W2033407116 doi "https://doi.org/10.1006/abbi.2000.2012" @default.
• W2033407116 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11051096" @default.
• W2033407116 hasPublicationYear "2000" @default.
• W2033407116 type Work @default.
• W2033407116 sameAs 2033407116 @default.
• W2033407116 citedByCount "22" @default.
• W2033407116 countsByYear W20334071162013 @default.
• W2033407116 countsByYear W20334071162014 @default.
• W2033407116 countsByYear W20334071162018 @default.
• W2033407116 crossrefType "journal-article" @default.
• W2033407116 hasAuthorship W2033407116A5011896624 @default.
• W2033407116 hasAuthorship W2033407116A5031000915 @default.
• W2033407116 hasAuthorship W2033407116A5051559153 @default.
• W2033407116 hasAuthorship W2033407116A5068857226 @default.
• W2033407116 hasAuthorship W2033407116A5085983408 @default.
• W2033407116 hasConcept C181199279 @default.
• W2033407116 hasConcept C184235292 @default.
• W2033407116 hasConcept C185592680 @default.
• W2033407116 hasConcept C2778102346 @default.
• W2033407116 hasConcept C2780610907 @default.
• W2033407116 hasConcept C48349386 @default.
• W2033407116 hasConcept C55493867 @default.
• W2033407116 hasConceptScore W2033407116C181199279 @default.
• W2033407116 hasConceptScore W2033407116C184235292 @default.
• W2033407116 hasConceptScore W2033407116C185592680 @default.
• W2033407116 hasConceptScore W2033407116C2778102346 @default.
• W2033407116 hasConceptScore W2033407116C2780610907 @default.
• W2033407116 hasConceptScore W2033407116C48349386 @default.
• W2033407116 hasConceptScore W2033407116C55493867 @default.
• W2033407116 hasIssue "1" @default.
• W2033407116 hasLocation W20334071161 @default.
• W2033407116 hasLocation W20334071162 @default.
• W2033407116 hasOpenAccess W2033407116 @default.
• W2033407116 hasPrimaryLocation W20334071161 @default.
• W2033407116 hasRelatedWork W1509077075 @default.
• W2033407116 hasRelatedWork W1573935390 @default.
• W2033407116 hasRelatedWork W1867329478 @default.
• W2033407116 hasRelatedWork W1964622351 @default.
• W2033407116 hasRelatedWork W2004481779 @default.
• W2033407116 hasRelatedWork W2007526154 @default.
• W2033407116 hasRelatedWork W2033407116 @default.
• W2033407116 hasRelatedWork W2041424665 @default.
• W2033407116 hasRelatedWork W2088608872 @default.
• W2033407116 hasRelatedWork W2132066544 @default.

• next