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• W2033433765 abstract "To test for possible toxicity of soybean lectin for salmon, the location of soybean agglutinin binding sites in the small intestinal brush border membranes (BBM) was determined quantitatively by an enzyme-linked lectin sorbent assay (ELLSA). The assay revealed substantial binding in both the proximal (Bmax 1764±170 nM per mg BBM protein; Kd 2.16±0.23·10−5) and distal (Bmax 2096±79 nM per mg BBM protein; Kd 1.58±0.11·10−5) small intestine. The higher maximum binding and the lower dissociation constant for the distal small intestine might indicate a greater sensitivity to the toxic effect of soybean lectin in this region. Enzyme activities for alkaline phosphatase (EC 3.1.3.1) and sucrase isomaltase (EC 3.2.1.48) in brush border membrane preparations were very low in both the proximal and distal small intestine. A high enzyme activity was found for aminopeptidase (EC 3.4.11.2). Activities of sucrase isomaltase and aminopeptidase were lower in the distal than in the proximal small intestine." @default.
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• W2033433765 date "1990-12-01" @default.
• W2033433765 modified "2023-10-14" @default.
• W2033433765 title "Binding of soybean agglutinin to small intestinal brush border membranes and brush border membrane enzyme activities in Atlantic salmon (Salmo salar)" @default.
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• W2033433765 doi "https://doi.org/10.1016/0044-8486(90)90185-p" @default.
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