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- W2033449465 abstract "Yeast and E . coli tRNA phe samples were oxidized and labeled at the 3′ end with dansy1 hydrazine or fluorescein thiosemicarbazide. These tRNAs can bind to poly(U)-programmed E . coli 70S tight couple ribosomes in 25 mM magnesium at 8°C. Two binding sites with binding constants of about 1 × 10°9 M − 1 (P) and 3×10 7 M −1 (A) were determined for the yeast tRNA phe derivatives, With E . coli tRNA phe the A site affinity is similar to yeast tRNA phe but the P site affinity is 5-fold weaker. Singlet-singlet energy transfer showed that the distance from the 3′ end of tRNA phe in the P site to a fluorescein derivative of erythromycin is 23 Å. This supports invitro studies suggesting that erythromycin binds near the peptide moiety of peptidyl tRNA. A distance of 34 Å between the 3′ ends of 2 tRNAs bound simultaneously on the ribosome was also measured. This long distance may mean that the deacylated fluorescent tRNA binds to the A site in an orientation like that in the stringent response rather than in protein synthesis." @default.
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- W2033449465 date "1980-01-01" @default.
- W2033449465 modified "2023-09-23" @default.
- W2033449465 title "Ribosome binding by tRNAs with fluorescent labeled 3′ termini" @default.
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- W2033449465 doi "https://doi.org/10.1093/nar/8.14.3229" @default.
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