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- W2033456218 abstract "Organophosphorus ester-induced delayed neurotoxicity (OPIDN) is a neurodegenerative disorder characterized by ataxia progressing to paralysis with a concomitant central and peripheral distal axonapathy. Diisopropylphosphorofluoridate (DFP) produces OPIDN in the chicken, which results in mild ataxia in 7-14 days and severe paralysis as the disease progresses with a single dose. White leghorn layer hens were treated with DFP (1.7 mg/kg, sc) after prophylactic treatment with atropine (1 mg/kg, sc) in normal saline and eserine (1 mg/kg, sc) in dimethyl sulfoxide. Control groups were treated with vehicle propylene glycol (0.1 mL/kg, sc), atropine in normal saline and eserine in dimethyl sulfoxide. The hens were sacrificed at different time points such as 2, 4, and 8 h, as well as 1, 2, 5, 10 and 20 days, and the tissues from cerebrum, midbrain, cerebellum brainstem and spinal cord were quickly dissected and frozen for protein (western) and mRNA (northern) studies. Subcellular fractionation, SDS-PAGE and immunoblotting of the nuclear and supernatant fractions using standard protocols from spinal cord and cerebrum showed differential expression of protein levels of PKA, CREB and phosphorylated CREB (p-CREB). There was an increase in PKA level in spinal cord nuclear fraction after 4 h (130+/-5%) and 8 h (133+/-6 %), while cerebrum nuclear fraction showed decrease (77+/-5%) at 4 h and remained at the same level at 8 h. No change was seen in either spinal cord or cerebrum soluble fraction at any time points. There was an increase in CREB level in the spinal cord supernatant (133+/-3%) after 5 days, while nuclear and supernatant fraction of the cerebrum did not show any alterations at any time point. p-CREB was induced in the spinal cord nuclear fraction at 1 day (150+/-3%) and 5 days (173+/-7%) of treatment, in contrast to the decreased levels p-CREB (72+/-4%) at 10 days in cerebrum nuclear fraction. Supernatant fraction of spinal cord and cerebrum did not show any changes in pCREB at time points studied. Similarly another set of animals were treated with DFP and perfused using standard protocols and immunohistochemistry for p-CREB in the brain and spinal cord confirmed the overall protein expression pattern identified by western analysis. Expression of beta-tubulin subtypes (1, 2, 3, and 4), studied by Northern blotting showed complex and differential pattern, while immunohistochemistry of the anti-beta-tubulin for the entire period of OPIDN developmental stages showed early induction and persistence even in the disintegrating axonal and non-neuronal structures of the CNS. These data thus strongly suggest that early cytoskeletal damage at molecular level mediated by PKA/p-CREB pathways leads to the culmination of gross (microscopically observable) level cytoskeletal changes in various components of central nervous system (CNS), consistent with our earlier findings. Thus, the differential protein expression of PKA, CREB, p-CREB and beta-tubulin subtypes appear to contribute to the initiation, progression and development of OPIDN, probably by recruiting other molecular pathways specific to various components of nervous system." @default.
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- W2033456218 date "2009-10-01" @default.
- W2033456218 modified "2023-10-16" @default.
- W2033456218 title "DFP initiated early alterations of PKA/p-CREB pathway and differential persistence of β-tubulin subtypes in the CNS of hens contributes to OPIDN" @default.
- W2033456218 cites W116527972 @default.
- W2033456218 cites W130618852 @default.
- W2033456218 cites W144167353 @default.
- W2033456218 cites W1532805410 @default.
- W2033456218 cites W1641477052 @default.
- W2033456218 cites W178343997 @default.
- W2033456218 cites W1972891081 @default.
- W2033456218 cites W1973327478 @default.
- W2033456218 cites W1977913342 @default.
- W2033456218 cites W1978300562 @default.
- W2033456218 cites W1983789487 @default.
- W2033456218 cites W1988726352 @default.
- W2033456218 cites W1990591057 @default.
- W2033456218 cites W1990735551 @default.
- W2033456218 cites W1993733923 @default.
- W2033456218 cites W1998148816 @default.
- W2033456218 cites W2010549737 @default.
- W2033456218 cites W2015878359 @default.
- W2033456218 cites W2019500590 @default.
- W2033456218 cites W2022372459 @default.
- W2033456218 cites W2038117045 @default.
- W2033456218 cites W2038431896 @default.
- W2033456218 cites W2038507834 @default.
- W2033456218 cites W2043016952 @default.
- W2033456218 cites W2051741478 @default.
- W2033456218 cites W2054267191 @default.
- W2033456218 cites W2058730899 @default.
- W2033456218 cites W2062796773 @default.
- W2033456218 cites W2064598388 @default.
- W2033456218 cites W2070018885 @default.
- W2033456218 cites W2070776710 @default.
- W2033456218 cites W2071140879 @default.
- W2033456218 cites W2076050540 @default.
- W2033456218 cites W2088057294 @default.
- W2033456218 cites W2088853316 @default.
- W2033456218 cites W2091693735 @default.
- W2033456218 cites W2094760990 @default.
- W2033456218 cites W2095092814 @default.
- W2033456218 cites W2095997832 @default.
- W2033456218 cites W2100837269 @default.
- W2033456218 cites W2103703726 @default.
- W2033456218 cites W2103745379 @default.
- W2033456218 cites W2106711938 @default.
- W2033456218 cites W2110724392 @default.
- W2033456218 cites W2113096380 @default.
- W2033456218 cites W2119795117 @default.
- W2033456218 cites W2132583383 @default.
- W2033456218 cites W2132691381 @default.
- W2033456218 cites W2132985423 @default.
- W2033456218 cites W2137701272 @default.
- W2033456218 cites W2138875437 @default.
- W2033456218 cites W2153870748 @default.
- W2033456218 cites W2165543524 @default.
- W2033456218 cites W2169975019 @default.
- W2033456218 cites W222254532 @default.
- W2033456218 cites W244606901 @default.
- W2033456218 cites W340865646 @default.
- W2033456218 cites W51180725 @default.
- W2033456218 cites W79984152 @default.
- W2033456218 doi "https://doi.org/10.1016/j.taap.2009.07.035" @default.
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