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• W2033459508 abstract "Polyethyleneimine (PEI) has been used previously as a nonviral DNA transfer vector. In this article, we demonstrate its use as a vehicle for transmembrane delivery of proteins in cell culture conditions. Linking proteins to PEI required no other treatment beyond mixing them with PEI. The bond between PEI and protein combined at optimal ratios was maintained in electrophoresis, even in the presence of 2.5% sodium dodecyl sulfate (SDS). The optimal time for delivery of proteins was determined to be 24 h. We have successfully delivered an Alexa 488-labeled avidin protein into human glioblastoma cells. A functional antibody against the nuclear protein lamin was delivered into human fibroblasts and reacted with lamin inside live cells. PEI-based delivery of antibodies and fluorescently labeled proteins can be used for fluorescent detection, tracking, and evaluation of cellular protein function in vivo." @default.
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• W2033459508 date "2005-09-01" @default.
• W2033459508 modified "2023-10-16" @default.
• W2033459508 title "Polyethyleneimine as a transmembrane carrier of fluorescently labeled proteins and antibodies" @default.
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• W2033459508 doi "https://doi.org/10.1016/j.ab.2005.06.011" @default.
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