FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033472906> ?p ?o ?g. }

• W2033472906 endingPage "240" @default.
• W2033472906 startingPage "231" @default.
• W2033472906 abstract "The aims of this study were to investigate steady-state level of Kit Ligand (KL) mRNA and its effects on in vitro survival and growth of caprine preantral follicles. RT-PCR was used to analyze caprine steady-state level of KL mRNA in primordial, primary, and secondary follicles, and in small (1–3 mm) and large (3–6 mm) antral follicles. Furthermore, ovarian fragments were cultured for 1 or 7 days in Minimal Essential Medium (MEM+) supplemented with KL (0, 1, 10, 50, 100, or 200 ng/ml). Noncultured (control) and cultured fragments were processed for histology and transmission electron microscopy (TEM). RT-PCR demonstrated an increase in steady-state level of KL mRNA during the transition from primary to secondary follicles. Small antral follicles had higher steady-state levels of KL mRNA in granulosa and theca cells than large follicles. After 7 days, only 50 ng/ml of KL had maintained the percentage of normal follicles similar to control. After 1 day, all KL concentrations reduced the percentage of primordial follicles and increased the percentage of growing follicles. KL at 10, 50, 100, or 200 ng/ml increased primary follicles, compared to MEM+ after 7 days. An increase in oocyte and follicular diameter was observed at 50 ng/ml of KL. TEM confirmed ultrastructural integrity of follicles after 7 days at 50 ng/ml of KL. In conclusion, the KL mRNAs were detected in all follicular categories. Furthermore, 50 ng/ml of KL maintained the integrity of caprine preantral follicle cultured for 7 days and stimulated primordial follicle activation and follicle growth. Mol. Reprod. Dev. 77: 231–240, 2010. © 2009 Wiley-Liss, Inc." @default.
• W2033472906 created "2016-06-24" @default.
• W2033472906 creator A5001858126 @default.
• W2033472906 creator A5007407541 @default.
• W2033472906 creator A5019226350 @default.
• W2033472906 creator A5021848943 @default.
• W2033472906 creator A5023210157 @default.
• W2033472906 creator A5027393058 @default.
• W2033472906 creator A5030869706 @default.
• W2033472906 creator A5037453553 @default.
• W2033472906 creator A5044404097 @default.
• W2033472906 creator A5046492044 @default.
• W2033472906 creator A5058199726 @default.
• W2033472906 creator A5059044986 @default.
• W2033472906 creator A5075252814 @default.
• W2033472906 creator A5084602669 @default.
• W2033472906 creator A5091311995 @default.
• W2033472906 date "2009-12-11" @default.
• W2033472906 modified "2023-10-16" @default.
• W2033472906 title "Steady-state level of kit ligand mRNA in goat ovaries and the role of kit ligand in preantral follicle survival and growth in vitro" @default.
• W2033472906 cites W1501791679 @default.
• W2033472906 cites W1864184149 @default.
• W2033472906 cites W1965369413 @default.
• W2033472906 cites W1965695331 @default.
• W2033472906 cites W1966188866 @default.
• W2033472906 cites W1968409042 @default.
• W2033472906 cites W1974757434 @default.
• W2033472906 cites W1987238854 @default.
• W2033472906 cites W1991050149 @default.
• W2033472906 cites W2009766889 @default.
• W2033472906 cites W2015527564 @default.
• W2033472906 cites W2018853148 @default.
• W2033472906 cites W2029218543 @default.
• W2033472906 cites W2033250990 @default.
• W2033472906 cites W2033482094 @default.
• W2033472906 cites W2036246831 @default.
• W2033472906 cites W2038725702 @default.
• W2033472906 cites W2042272294 @default.
• W2033472906 cites W2042556223 @default.
• W2033472906 cites W2049004724 @default.
• W2033472906 cites W2055219121 @default.
• W2033472906 cites W2062763831 @default.
• W2033472906 cites W2076644582 @default.
• W2033472906 cites W2078705189 @default.
• W2033472906 cites W2079265019 @default.
• W2033472906 cites W2084241850 @default.
• W2033472906 cites W2091313483 @default.
• W2033472906 cites W2096214136 @default.
• W2033472906 cites W2097224108 @default.
• W2033472906 cites W2097425334 @default.
• W2033472906 cites W2110274446 @default.
• W2033472906 cites W2119029365 @default.
• W2033472906 cites W2125564527 @default.
• W2033472906 cites W2129072528 @default.
• W2033472906 cites W2138965475 @default.
• W2033472906 cites W2139417605 @default.
• W2033472906 cites W2143932116 @default.
• W2033472906 cites W2147629399 @default.
• W2033472906 cites W2150921965 @default.
• W2033472906 cites W2151211077 @default.
• W2033472906 cites W2155361997 @default.
• W2033472906 cites W2155401408 @default.
• W2033472906 cites W2156683634 @default.
• W2033472906 cites W2162905148 @default.
• W2033472906 cites W2168768523 @default.
• W2033472906 cites W2171808136 @default.
• W2033472906 doi "https://doi.org/10.1002/mrd.21138" @default.
• W2033472906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20014130" @default.
• W2033472906 hasPublicationYear "2009" @default.
• W2033472906 type Work @default.
• W2033472906 sameAs 2033472906 @default.
• W2033472906 citedByCount "40" @default.
• W2033472906 countsByYear W20334729062012 @default.
• W2033472906 countsByYear W20334729062013 @default.
• W2033472906 countsByYear W20334729062014 @default.
• W2033472906 countsByYear W20334729062015 @default.
• W2033472906 countsByYear W20334729062016 @default.
• W2033472906 countsByYear W20334729062018 @default.
• W2033472906 countsByYear W20334729062020 @default.
• W2033472906 countsByYear W20334729062021 @default.
• W2033472906 countsByYear W20334729062023 @default.
• W2033472906 crossrefType "journal-article" @default.
• W2033472906 hasAuthorship W2033472906A5001858126 @default.
• W2033472906 hasAuthorship W2033472906A5007407541 @default.
• W2033472906 hasAuthorship W2033472906A5019226350 @default.
• W2033472906 hasAuthorship W2033472906A5021848943 @default.
• W2033472906 hasAuthorship W2033472906A5023210157 @default.
• W2033472906 hasAuthorship W2033472906A5027393058 @default.
• W2033472906 hasAuthorship W2033472906A5030869706 @default.
• W2033472906 hasAuthorship W2033472906A5037453553 @default.
• W2033472906 hasAuthorship W2033472906A5044404097 @default.
• W2033472906 hasAuthorship W2033472906A5046492044 @default.
• W2033472906 hasAuthorship W2033472906A5058199726 @default.
• W2033472906 hasAuthorship W2033472906A5059044986 @default.
• W2033472906 hasAuthorship W2033472906A5075252814 @default.
• W2033472906 hasAuthorship W2033472906A5084602669 @default.
• W2033472906 hasAuthorship W2033472906A5091311995 @default.
• W2033472906 hasConcept C102570824 @default.

• next